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http://dx.doi.org/10.5352/JLS.2015.25.4.376

Construction of Ovine Customer cDNA Chip and Analysis of Gene Expression Patterns in the Muscle and Fat Tissues of Native Korean Cattle  

Han, Kyung Ho (Department of Cell and Molecular Biology, Scripps Research Institute)
Choi, Eun Young (Department of Biological Science, Silla University)
Hong, Yeon-Hee (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Kim, Jae Yeong (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Choi, In Soon (Department of Biological Science, Silla University)
Lee, Sang-Suk (Department of Animal Science and Technology, Sunchon National University)
Choi, Yun Jaie (School of Agricultural Biotechnology, Seoul National University)
Cho, Kwang Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.25, no.4, 2015 , pp. 376-384 More about this Journal
Abstract
To investigate the molecular events of controlling intramuscular fat (or marbling), which is an important factor in the evaluation of beef quality, we performed cDNA microarray analyses using the longissimus dorsi muscle and back fat tissues. For this study, we constructed normalized cDNA libraries: fat tissues in native Korean cattle (displaying 1,211 specific genes), and muscle tissues in native Korean cattle (displaying 1,346 specific genes). A bovine cDNA chip was constructed with 1,680 specific genes, consisting of 760 genes from muscle tissues and 920 genes from fat tissues. The microarray analysis in this experiment showed a number of differentially expressed genes, which compared the longissimus dorsi muscle (Cy5) with back fat tissue (Cy3). Among many specific differentially expressed genes, 12-lipoxygenase (oxidizing esterified fatty acids) and prostaglandin D synthase (differentiation of fibroblasts to adipocytes) are the key candidate enzymes that should be involved in controlling the accumulation of intramuscular fat. In this study, differentially and commonly expressed genes in the muscle and fat tissues of native Korean cattle were found in large numbers, using the hybridization assay. The expression levels of the selected genes were confirmed by semi-quantitative RT-PCR, and the results were similar to those of the cDNA microarray.
Keywords
Back fat tissue; cDNA microarray; longissimus dorsi muscle; native Korean cattle; rump tissue;
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