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http://dx.doi.org/10.5352/JLS.2015.25.3.345

Organic Solvent Stable Lipase from Pseudomonas sp. BCNU 171  

Choi, Hye Jung (Department of Biology, Changwon National University)
Kwon, Gi-Seok (Department of Bioresource Science, Andong National University)
Joo, Woo Hong (Department of Biology, Changwon National University)
Publication Information
Journal of Life Science / v.25, no.3, 2015 , pp. 345-348 More about this Journal
Abstract
An organic solvent stable lipase from solvent-tolerant Pseudomonas sp. BCNU 171 had an optimal pH of 8 and an optimal temperature of 37℃. This crude extracellular lipase from BCNU 171 exhibited increased stability in the presence of various types of solvents at high concentrations (25%, v/v). The lipase stability was found to be highest in the presence of xylene (137%), followed by toluene (131%), octane (130%), and butanol (104%). Overall, BCNU 171 lipase tended to be more stable than immobilized commercial lipase (Novozyme435) in the presence of organic solvents. Furthermore, BCNU 171 lipase maintained about 90% of its enzyme original activity in the presence of NH4+, Na+, Ba2+, Hg2+, Ni2+, Cu2+, and Ca2+ion and significantly increased its enzyme activity in the presence of various emulsifying agents. Thus, the organic solvent stable lipase from Pseudomonas sp. BCNU 171 could be usable as a potential whole cell biocatalyst and for synthetic applications of enzymes for industrial chemical processes in organic solvents without using immobilization.
Keywords
Lipase stability; organic solvent stable lipase; organic solvent tolerant bacterium Pseudomonas sp.;
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