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http://dx.doi.org/10.5352/JLS.2015.25.3.293

Ethanol Extract of Schisandra chinensis (Turcz.) Baill. Reduces AICAR-induced Muscle Atrophy in C2C12 Myotubes  

Kang, Young-Soon (Department of Biochemistry, Dongeui University College of Korean Medicine)
Park, Cheol (Department of Biochemistry, Dongeui University College of Korean Medicine)
Han, Min-Ho (Department of Molecular Biology, College of Natural Sciences & Human Ecology, Dongeui University)
Hong, Su-Hyun (Department of Biochemistry, Dongeui University College of Korean Medicine)
Hwang, Hye-Jin (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Kim, Byung Woo (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Kim, Cheol Min (Department of Biochemistry, Busan National University College of Medicine)
Choi, Yung Hyun (Department of Biochemistry, Dongeui University College of Korean Medicine)
Publication Information
Journal of Life Science / v.25, no.3, 2015 , pp. 293-298 More about this Journal
Abstract
Muscle atrophy, known as a sarcopenia, is defined as a loss of muscle mass resulting from a reduction in the muscle fiber area or density due to a decrease in muscle protein synthesis and an increase in protein breakdown. Schisandrae fructus (SF) extract of the fruits of Schisandra chinensis (Turcz) Baillon has been used as a tonic in traditional medicine for thousands of years. Although a great deal of work has been carried out on the therapeutic potential of SF, its pharmacological mechanisms of action in muscle diseases actions remain unclear. In the present study, we investigated the inhibitory effects of SF ethanol extracts on the production of muscle atrophy factors in C2C12 myotubes stimulated with 5-aminoimidazole-4-carboxamide-ribonucleotide (AICAR), an AMP-activated kinase (AMPK) activator, and sought to determine the underlying mechanisms of action. AICAR upregulated atrophy-related ubiquitin ligase muscle RING finger-1 (MuRF-1) and stimulated the levels of the forkhead box O3a (FoxO3a) transcription factor in the C2C12 myotubes. SF supplementation effectively and concentration- dependently counteracted AICAR-induced muscle cell atrophy and reversed the increased expression of MuRF-1 and FoxO3a. Our study demonstrates that SF can reverse the muscle cell atrophy caused by AICAR through regulation of the AMPK and FoxO3a signaling pathways, followed by inhibition of MuRF-1.
Keywords
AICAR (5-aminoimidazole-4-carboxamide-ribonucleotide); FoxO3a (forkhead box O3a); muscle atrophy; MuRF-1 (muscle RING finger-1); Schisandrae Fructus;
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