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http://dx.doi.org/10.5352/JLS.2015.25.1.93

Induction of Apoptosis by Pachymic Acid in T24 Human Bladder Cancer Cells  

Jeong, Jin-Woo (Division of Applied Life Science (BK21 Plus), Gyeongsang National University)
Baek, Jun Young (Department of Microbiology, College of Natural Sciences, Pusan National University)
Kim, Kwang Dong (Division of Applied Life Science (BK21 Plus), Gyeongsang National University)
Choi, Yung Hyun (Department of Biochemistry, Dong-Eui University College of Korean Medicine)
Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.25, no.1, 2015 , pp. 93-100 More about this Journal
Abstract
Pachymic acid (PA) is a lanostane-type triterpenoid derived from the Poria cocos mushroom. Several beneficial biological features of PA provide medicine with a wide variety of valuable effects, such as anticancer and anti-inflammatory activity; it also has antioxidant effects against oxidative stress. Nonetheless, the biological properties and mechanisms that produce this anti-cancer action of PA remain largely undetermined. In this study, we investigated the pro-apoptotic effects of PA in T24 human bladder cancer cells. It was found that PA could inhibit the cell growth of T24 cells in a dose-dependent manner, which was associated with the induction of apoptotic cell death, as evidenced by the formation of apoptotic bodies and chromatin condensation and accumulation of cells in the sub-G1 phase. The induction of apoptotic cell death by PA was connected with an up-regulation of pro-apoptotic Bax and Bad protein expression and down-regulation of anti-apoptotic Bcl-2 and Bcl-xL proteins, and inhibition of apoptosis family proteins. In addition, apoptosis-inducing concentrations of PA induced the activation of caspase-9, an initiator caspase of the mitochondrial-mediated intrinsic pathway, and caspase-3, accompanied by proteolytic degradation of poly (ADP-ribose)-polymerase. PA also induced apoptosis via a death receptor-mediated extrinsic pathway by caspase-8 activation, resulting in the truncation of Bid and suggesting the existence of cross-talk between the extrinsic and intrinsic pathways. Taken together, the present results suggest that PA may be a potential chemotherapeutic agent for the control of human bladder cancer cells.
Keywords
Apoptosis; bladder cancer cells; caspase; pachymic acid (PA); tBid;
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