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http://dx.doi.org/10.5352/JLS.2014.24.9.967

Anti-proliferative Activities of Solvent Fractions of Lees Extracts in Human Colorectal HCT116 Cells  

Kang, Hyung-Taek (Department of Biological Sciences, Andong National University)
Lee, Seung Hoon (Department of Biological Sciences, Andong National University)
Kim, Soon Young (Department of Biological Sciences, Andong National University)
Kim, Mi-Sun (Department of Food and Nutrition, Andong National University)
Shin, Woo-Chang (Research Institute, Kooksoondang Brewery Co., Ltd.)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Kim, Jong-Sik (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.24, no.9, 2014 , pp. 967-972 More about this Journal
Abstract
In the present study, we prepared eighty-five different kinds of lees extracts and their solvent fractions and investigated their anti-proliferative activities against human colorectal cancer HCT116 cells. HCT116 cells were treated with eighty-five solvent fractions of lees extracts and then cell viability was measured using MTS assay. Among the treated solvent fractions, three solvent fractions (KSD-E1-3, KSD-E2-3, and KSD-E4-3) were selected based on cell viability assay. In addition, we performed an oligo DNA microarray analysis to analyze the gene expression changes by treatment of KSD-E1-3 in HCT116 cells. Among the upregulated genes, we selected 4 genes (NAG-1, ATF3, p21, and DDIT3) and performed RT-PCR using gene-specific primers. Among the treated solvent fractions, KSD-E1-3 dramatically induced the expressions of the four selected genes. In addition, we investigated whether the upregulations of those genes were dependent on the transcription factor p53's presence using p53 null HCT116 cells. The results indicate that the upregulations of NAG-1, ATF3, and DDIT3 are not dependent on the p53 presence, whereas p21 is dependent on the p53 presence. These findings may help to understand the molecular mechanisms of the anti-proliferative activity mediated by rice wine lees in human colorectal cancer cells.
Keywords
Anti-proliferation; HCT116 cells; lees; oligo DNA microarray; p53;
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