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http://dx.doi.org/10.5352/JLS.2014.24.9.1012

Apoptotic Effect of Sasa quelpaertensis Nakai in Human Colon Cancer HT-29 Cells  

Byun, Ji Hee (Toxicology Laboratory, Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University)
Kim, Min Young (Toxicology Laboratory, Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University)
Publication Information
Journal of Life Science / v.24, no.9, 2014 , pp. 1012-1018 More about this Journal
Abstract
Sasa quelpaertensis Nakai (Korean name, Jeju-Joritdae) is one of the most abundant plants on Mt. Halla, Jeju Island, and it has long been used in traditional medicines. Recent studies have reported it as possessing various beneficial functions, including anti-inflammatory, anti-diabetic, anti-hypertension, anti-gastritis, anti-oxidant, and anti-cancer effects. However, the molecular mechanisms of its anti-cancer activity have not been clearly elucidated. In this study, we investigated the anti-cancer effects and mechanism of S. quelpaertensis on human colon cancer HT-29 cells. Cell growth inhibition by S. quelpaertensis was determined by MTT assay. Apoptosis was performed by DNA fragmentation, flow cytometry with propidium iodide staining (PI), and reverse transcription-polymerase chain reaction (RT-PCR) to confirm the anti-apoptotic factors, such as inhibitor of apoptosis (IAP) family members. $NO^{\bullet}$ production was determined by Griess assay. S. quelpaertensis treatment resulted in the time- and dose-dependent inhibition of the cell viability of HT-29 cells by inducing apoptosis, as evidenced by the accumulation of the sub-G1 cell population stained by PI, as well as the ladder-like DNA fragmentation in a dose-dependent manner. S. quelpaertensis-inducing apoptosis was accompanied by the induction of S cell cycle arrests, increasing $NO^{\bullet}$ concentrations, and the down-regulation of IAPs, including X-chromosome-linked IAP (XIAP), cellular IAP-1 (cIAP-1), cIAP-2, and survivin. Taken together, these findings have important implications for future clinical developments of S. quelpaertensis in colon cancer treatment.
Keywords
Apoptosis; HT-29 cells; IAP family; nitric oxide; Sasa quelpaertensis Nakai;
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Times Cited By KSCI : 11  (Citation Analysis)
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