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http://dx.doi.org/10.5352/JLS.2014.24.8.887

Development of Microbial Augmentation for the Treatment of Recalcitrant Industrial Wastewater Containing Chlorinated Organic Compounds  

Lee, Hyun Don (Department of Evironmental Engineering, Gyeongnam National University of Science and Technology)
Im, Seong Won (Department of Evironmental Engineering, Gyeongnam National University of Science and Technology)
Suh, Hyun-Hyo (Department of Evironmental Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.24, no.8, 2014 , pp. 887-894 More about this Journal
Abstract
The screening of the microorganisms degrading chlorinated organic compounds such as PCP (pentachlorophenol) and TCE (trichloroethylene) was conducted with soil and industrial wastewater contaminated with various chlorinated organic compounds. Isolates (GP5, GP19) capable of degrading PCP and isolates (GA6, GA15) capable of degrading TCE were identified as Acetobactor sp., Pseudomonas sp., Arthrobacer sp., Xanthomonas sp. and named Acetobacter sp. GP5, Pseudomonas sp. GP19, Arthrobacer sp. GA6 and Xanthomoas sp. GA15, respectively. The microbial augmentation, OC17 formulated with the mixture of bacteria including isolates (4 strains) degrading chlorinated organic compounds and isolates (Acinetobacter sp. KN11, Neisseria sp. GN13) degrading aromatic hydrocarbons. Characteristics of microbial augmentation OC-17 showed cell mass of $2.8{\times}10^9CFU/g$, bulk density of $0.299g/cm^3$ and water content of 26.8%. In the experiment with an artificial wastewater containing PCP (500 mg/l), degradation efficiency of the microbial augmentation OC17 was 87% during incubation of 65 hours. The degradation efficiency of TCE (300 uM) by microbial augmentation OC17 was 90% during incubation of 50 hours. In a continuous culture experiment, analysis of the biodegradation of organic compounds by microbial augmentation OC17 in industry wastewater containing chlorinated hydrocarbons showed that the removal rate of COD was 91% during incubation of 10 days. These results indicate that it is possible to apply the microbial augmentation OC17 to industrial wastewaters containing chlorinated organic compounds.
Keywords
Chemical oxygen demand; chlorinated organic compounds; continuous culture; microbial augmentation; wastewater treatment;
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