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http://dx.doi.org/10.5352/JLS.2014.24.8.820

Parkin Interacts with the PDZ Domain of Multi-PDZ Domain Protein MUPP1  

Jang, Won Hee (Departments of Biochemistry and u-HARC, Inje University College of Medicine)
Jeong, Young Joo (Departments of Biochemistry and u-HARC, Inje University College of Medicine)
Choi, Sun Hee (Departments of Biochemistry and u-HARC, Inje University College of Medicine)
Lee, Won Hee (Departments of Neurosurgery, Inje University College of Medicine)
Kim, Mooseong (Departments of Neurosurgery, Inje University College of Medicine)
Kim, Sang-Jin (Departments of Neurology, Inje University College of Medicine)
Urm, Sang-Hwa (Departments of Preventive Medicine, Inje University College of Medicine)
Moon, Il Soo (Departments of Anatomy & Dongguk Medical Institute, College of Medicine, Dongguk University)
Seog, Dae-Hyun (Departments of Biochemistry and u-HARC, Inje University College of Medicine)
Publication Information
Journal of Life Science / v.24, no.8, 2014 , pp. 820-826 More about this Journal
Abstract
The localization to specific subcellular sites and the regulation of cell surface receptors and channels are crucial for proper functioning. Postsynaptic density-95/Disks large/Zonula occludens-1 (PDZ)-domain is involved in recognition of and interaction between various proteins, by which the localization and the regulation are mediated. Multi-PDZ domain protein 1 (MUPP1) contains 13 PDZ domains. MUPP1 serves a scaffolding function for structure proteins and signaling proteins, but the mechanism how MUPP1 is stabilized and signalized has not yet been elucidated. We used the yeast two-hybrid system to identify proteins that interact with PDZ domains of MUPP1. We found an interaction between MUPP1 and Parkin. Parkin is an E3 ubiquitin ligase. Loss-of-function mutations of Parkin gene are known to cause an autosomal recessive juvenile parkinsonism. Parkin bound to the $12^{th}$ PDZ domain, but not to other PDZ domains of MUPP1. The C-terminal end of Parkin has a type II PDZ-association motif, which was essential for the interaction with MUPP1 in the yeast two-hybrid assay. When co-expressed in HEK-293T cells, Parkin co-localized with MUPP1. When co-expressed with ubiquitin in HEK-293T cells, MUPP1 has been strongly ubiquitinated by Parkin. These findings collectively suggest that MUPP1 is a novel substrate of Parkin and its function or stability could be modulated by Parkin-mediated ubiquitination.
Keywords
MUPP1; Parkin; PDZ domain; protein-protein interaction; ubiquitination;
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1 Becamel, C., Figge, A., Poliak, S., Dumuis, A., Peles, E., Bockaert, J., Lubbert, H. and Ullmer, C. 2001. Interaction of serotonin 5-hydroxytryptamine type 2C receptors with PDZ10 of the multi-PDZ domain protein MUPP1. J Biol Chem 276, 12974-12982.   DOI   ScienceOn
2 Adachi, M., Hamazaki, Y., Kobayashi, Y., Itoh, M., Tsukita, S., Furuse, M. and Tsukita, S. 2009. Similar and distinct properties of MUPP1 and Patj, two homologous PDZ domain-containing tight-junction proteins. Mol Cell Biol 29, 2372-2389.   DOI   ScienceOn
3 Balasubramanian, S., Fam, S. R. and Hall, R. A. 2007. GABAB receptor association with the PDZ Scaffold Mupp1 alters receptor stability and function. J Biol Chem 282, 4162-4171.
4 Coelln, R., Valina, L. and Dawson, T. M. 2004. Parkin-associated Parkinson's disease. Cell Tissue Res 318, 175-184.   DOI   ScienceOn
5 Dev, K. K., van der Putten, H., Sommer, B. and Rovelli, G. 2003. Part I: parkin-associated proteins and Parkinson's disease. Neuropharmacology 45, 1-13.   DOI   ScienceOn
6 Dev, K. K. 2004. Making protein interactions druggable: targeting PDZ domains. Nat Rev Drug Discov 3, 1047-1056.   DOI   ScienceOn
7 Doyle, D. A., Lee, A., Lewis, J., Kim, E., Sheng, M. and MacKinnon, R. 1996. Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ. Cell 85, 1067-1076.   DOI   ScienceOn
8 Field, C. M. and Kellogg, D. 1999. Septins: cytoskeletal polymers or signaling GTPases? Trends Cell Biol 9, 387-394.   DOI   ScienceOn
9 Garner, C. C., Nash, J. and Huganir, R. L. 2000. PDZ domains in synapse assembly and signalling. Trends Cell Biol 10, 274-280.   DOI   ScienceOn
10 Hamazaki, Y., Itoh, M., Sasaki, H., Furuse, M. and Tsukita, S. 2002. Multi-PDZ domain protein 1 (MUPP1) is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule. J Biol Chem 277, 455-461.   DOI   ScienceOn
11 Gomperts, S. N. 1996. Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84, 659-662.   DOI   ScienceOn
12 Guillaume, J. L., Daulat, A. M., Maurice, P., Levoye, A., Migaud, M., Brydon, L., Malpaux, B., Borg-Capra, C. and Jockers, R. 2008. The PDZ protein mupp1 promotes Gi coupling and signaling of the Mt1 melatonin receptor. J Biol Chem 283, 16762-16771.   DOI   ScienceOn
13 Guillemot, L., Foglia, A., Paschoud, S., Pulimeno, P. and Citi, S. 2008. The cytoplasmic plaque of tight junctions: a scaffolding and signalling center. Biochim Biophys Acta 1778, 601-613.   DOI   ScienceOn
14 Hershko, A. and Ciechanover, A. 1992. The ubiquitin system for protein degradation. Annu Rev Biochem 61, 761-807.   DOI   ScienceOn
15 Hirbec, H., Perestenko, O., Nishimune, A., Meyer, G., Nakanishi, S. and Henley, J. M. 2002. The PDZ proteins PICK1, GRIP and Syntenin bind multiple glutamate receptor subtypes. J Biol Chem 277, 15221-15224.   DOI   ScienceOn
16 Imai, Y., Soda, M. and Takahashi, R. 2000. Parkin suppresses unfolded protein stress-induced cell death through its E3 ubiquitin-protein ligase activity. J Biol Chem 275, 35661-35664.   DOI   ScienceOn
17 Leonoudakis, D., Conti, L. R., Radeke, C. M., McGuire, L. M. and Vandenberg, C. A. 2004. A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels. J Biol Chem 279, 19051-19063.   DOI   ScienceOn
18 Kimber, W. A., Trinkle-Mulcahy, L., Cheung, P., Deak, M., Marsden, L. J. and Kieloch, A. 2002. Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo. Biochem J 361, 525-536.   DOI
19 Kitada, T., Asakawa, S., Hattori, N., Matsumine, H., Yamamura, Y. and Minoshima, S. 1998. Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism. Nature 392, 605-608.   DOI   ScienceOn
20 Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual. Cold Spring Habor Laboratory, Cold Spring Habor, New York.
21 Lucking, C. B., Durr, A., Bonifati, V., Vaughan, J., De Michele, G. and Gasser, T. 2000. Association between early- onset Parkinson's disease and mutations in the parkin gene. French Parkinson's Disease Genetics Study Group. N Engl J Med 342, 1560-1567.   DOI   ScienceOn
22 Ponting, C. P., Phillips, C., Davies, K. E. and Blake, D. J. 1997. PDZ domains: targeting signalling molecules to submembranous sites. Bioessays 19, 469-479.   DOI   ScienceOn
23 Sheng, M. and Sala, C. 2001. PDZ domains and the organization of supramolecular complexes. Annu Rev Neurosci 24, 1-29.   DOI   ScienceOn
24 Shimura, H., Hattori, N., Kubo, S., Mizuno, Y., Asakawa, S. and Minoshima, S. 2000. Familial Parkinson disease gene product, parkin, is a ubiquitinprotein ligase. Nat Genet 25, 302-305.   DOI   ScienceOn
25 Ullmer, C., Schmuck, K., Figge, A. and Luebbert, H. 1998. Cloning and characterization of MUPP1, a novel PDZ domain protein. FEBS Lett 424, 63-68.   DOI   ScienceOn
26 Sitek, B., Poschmann, G., Schmidtke, K., Ullmer, C., Maskri, L. and Andriske, M. 2003. Expression of MUPP1 protein in mouse brain. Brain Research 970, 178-187.   DOI   ScienceOn
27 Songyang, Z., Fanning, A. S., Fu, C., Xu, J., Marfatia, S. M. and Chishti, A. H. 1997. Recognition of unique carboxyl-terminal motifs by distinct PDZ domains. Science 275, 73-77.   DOI   ScienceOn
28 Takeda, S., Yamazaki, H., Seog, D. H., Kanai, Y., Terada, S. and Hirokawa, N. 2000. Kinesin superfamily protein 3 (KIF3) motor transports fodrin-associating vesicles important for neurite building. J Cell Biol 148, 1255-1265.   DOI   ScienceOn
29 Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. 1998. Current Protocols in Molecular Biology. John Wiley & Sons.
30 Zhang, Y., Gao, J., Chung, K. K., Huang, H., Dawson, V. L. and Dawson, T. M. 2000. Parkin functions as an E2- dependent ubiquitin-protein ligase and promotes the degradation of the synaptic vesicle associated protein, CDCrel-1. Proc Natl Acad Sci USA 97, 13354-13359.   DOI   ScienceOn