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http://dx.doi.org/10.5352/JLS.2014.24.7.757

Antibacterial Effect of Bacteria Isolated from the Plant Rhizosphere against Pathogenic Bacteria of Fish  

Jeong, Ji-Woon (Major of Aquatic Life Medicine, Faculty of Marine Biomedical Sciences, Jeju National University)
Park, So-Hyun (Major of Aquatic Life Medicine, Faculty of Marine Biomedical Sciences, Jeju National University)
Kim, Dong-Hwi (Major of Aquatic Life Medicine, Faculty of Marine Biomedical Sciences, Jeju National University)
Jeun, Yong-Chull (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, Jeju National University)
Heo, Moon-Soo (Major of Aquatic Life Medicine, Faculty of Marine Biomedical Sciences, Jeju National University)
Publication Information
Journal of Life Science / v.24, no.7, 2014 , pp. 757-761 More about this Journal
Abstract
Olive flounder (Paralichthys olivaceus) is an important aquaculture fish species in Jeju Island, South Korea. Due to the intensification of flounder fish farming, huge amounts of chemical antibiotics are used against several fish diseases. This has many harmful side effects on fish, as well as human consumers. Hence, an alternative to chemical antibiotic agents is needed for disease control. In this study, three strains of rhizobacteria (BRH433-2, TRH415-2, and THJ609-3) were isolated from the rhizosphere of plants. Assays of their antibacterial activity against fish pathogens, such as S. iniae, S. parauberis, V. anguillarum, and E. tarda, were performed with untreated broth culture (without cell separation), supernatant, and precipitated pellets separated by centrifugation. Among these, the cell suspension prepared from the precipitated pellet showed significant antimicrobial activity when compared with that of the untreated broth culture and centrifugal supernatant. These results indicate that the three isolated rhizobacterial strains exhibit antibacterial activity. Analysis of the 16S rDNA sequences of the BRH-433-2, THJ609-3, and TRH415-2 strains showed the highest similarity to Burkholderia gladioli (99.5%), Pseudomonas baetica (97.7%), and P. koreensis and P. baetica (98.4%), respectively. We suggest that the strains hold promise in disease management of fish.
Keywords
Antibacterial activity; cell suspension; fish pathogens; phylogenetic tree; rhizobacteria;
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