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http://dx.doi.org/10.5352/JLS.2014.24.6.694

Characterization Study of Crude Oil Degrading Microbiology Isolated from Incheon Bay  

Choi, Hye Jin (Incheon Research Institute of Public Health and Environment)
Oh, Bo Young (Incheon Research Institute of Public Health and Environment)
Han, Young Sun (Incheon Research Institute of Public Health and Environment)
Hur, Myung Je (Incheon Research Institute of Public Health and Environment)
Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Journal of Life Science / v.24, no.6, 2014 , pp. 694-699 More about this Journal
Abstract
Indigenous microorganisms play decisive roles in biodegradation. In this study, eighty strains of hydrocarbon-degrading microbes were isolated from Incheon Bay. Among them, 12 strains were selected by an oil film collapsing method. The bacterial strain 'Incheon9' was eventually selected based on its relatively higher lipase and emulsification activities, and was identified as Acinetobacter sp. (NCBI accession code: KF54854). The optimum condition for the growth and emulsification activity of Acinetobacter sp. Incheon9 was $20^{\circ}C$, pH 7, and 1% NaCl. The optimum time for the best production of biosurfactant was 72 hrs. The oil degradation ability of Acinetobacter sp. Incheon9 was investigated by measuring the residual oils in the culture medium by gas chromatography (FID). This research provides foundational data for eco-friendly environmental remediation by microorganisms.
Keywords
Acinetobacter sp.; bioemulsifier; biosurfactant; lipase; oil-degradation;
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