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http://dx.doi.org/10.5352/JLS.2014.24.5.485

Whitening Effect of Poria cocas Ethanol Extract by Inhibition of Melanin Synthesis  

Park, Hye-Jung (Department of Chemistry, Dong-Eui University)
Kwon, Eun-Jeong (Department of Chemistry, Dong-Eui University)
Kim, Moon-Moo (Department of Chemistry, Dong-Eui University)
Lee, Kyeong Rok (AMI cosmetics)
Hong, Il (AMI cosmetics)
Lee, Do Gyeong (AMI cosmetics)
Oh, Yunghee (Department of Chemistry, Dong-Eui University)
Publication Information
Journal of Life Science / v.24, no.5, 2014 , pp. 485-490 More about this Journal
Abstract
Poria cocas has been reported to be effective in skin whitening. However, the direct effect of P. cocas ethanol extracts (PCEE) on melanin synthesis has not been scientifically studied. To elucidate the direct effect of PCEE on melanogenesis, a 3,4-dihydroxyindole-2-carboxylic acid (DOPA) synthesis assay, tyrosinase activity assay, and Western blotting for melanogenic proteins, including tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 were performed in mouse B16F1 cells. The results revealed that PGEE inhibited melanin production in a dose-dependent manner by blocking the synthesis of DOPA. Although the activation of tyrosinase was not affected, the expression levels of TRP-1 and TRP-2 were controlled. These results suggest that PCEE has a whitening effect, indicating that it may be a useful agent in the development of whitening cosmetics.
Keywords
B16F1; DOPA; Melanin; Poria cocas; tyrosinase-related protein (TRP)-1; TRP-2;
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