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http://dx.doi.org/10.5352/JLS.2014.24.3.329

Comparison of Anti-inflammatory Activities among Ethanol Extracts of Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus, and their Mixtures in RAW 246.7 Murine Macrophages  

Han, Min Ho (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Lee, Moon Hee (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Hong, Su Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Choi, Yung Hyun (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Moon, Ju Sung (Lioele Cosmetic Co., LTD.)
Song, Myung Kyu (Lioele Cosmetic Co., LTD.)
Kim, Min Ju (Lioele Cosmetic Co., LTD.)
Shin, Su Jin (Lioele Cosmetic Co., LTD.)
Hwang, Hye Jin (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Publication Information
Journal of Life Science / v.24, no.3, 2014 , pp. 329-335 More about this Journal
Abstract
Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus have been widely used in folk medicine for several inflammatory disorders in Korea and China. In this study, we compared the anti-inflammatory effects of the ethanol extracts of S. flavescens (EESF), G. uralensis (EEGU) and D. dasycarpus (EEDS), and their mixtures (medicinal herber mixtures, MHMIXs) on production of inflammatory mediators and cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages. Our data indicated that treatment with EESF, EEGU and EEDD significantly inhibited the excessive production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) in LPS-stimulated RAW 264.7 cells. The ethanol extracts and MHMIXs also attenuated the production of pro-inflammatory cytokines, including interleukin-$1{\beta}$ ($IL-1{\beta}$) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) by suppressing their protein expression, respectively. Interestingly, MHMIX-1, which basic ingredients are EESF, EEGU and EEDS in the proportion 3:1:1, more safely and effectively inhibits the LPS-induced inflammatory status in LPS-stimulated RAW 264.7 macrophages compared to ethanol extracts of each medicinal herb and other MHMIXs without causing any cytotoxic effects. Our study provides scientific evidence to support that a berbal mixture, MHMIX-1 may be useful in the treatment of inflammatory diseases by inhibiting inflammatory regulator responses in activated macrophages.
Keywords
Cytokine; inflammation; medicinal herbal mixtures; nitric oxide (NO); prostaglandin $E_2$ ($PGE_2$);
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