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http://dx.doi.org/10.5352/JLS.2014.24.3.323

Transcriptional Regulation of MicroRNA-17 by PPARγ in Adipogenesis  

Bae, In-Seon (Department of Biology, Kyung Hee University)
Kim, Hyun-Ji (Department of Biology, Kyung Hee University)
Chung, Ki Yong (Hanwoo Experiment Station, National Institute of Animal Science, RDA)
Choi, Inho (School of Biotechnology and Bovine Genome Resources Bank, Yeungnam University)
Kim, Sang Hoon (Department of Biology, Kyung Hee University)
Publication Information
Journal of Life Science / v.24, no.3, 2014 , pp. 323-328 More about this Journal
Abstract
MicroRNAs comprise a family of small noncoding RNAs that modulate physiological processes, including adipogenesis. MicroRNA-17 (miR-17) promotes adipocyte differentiation and enhances lipid accumulation. The transcriptional regulation of miR-17 during adipogenesis remains unknown. In this study, we investigated whether miR-17 is a target of peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$), which is a key regulator of adipogenesis. The levels of miR-17 and the expression of $PPAR{\gamma}$ increased after the induction of adipocyte differentiation. Three putative peroxisome proliferator response elements (PPREs) were identified in the miR-17 promoter region. Using chromatin immunoprecipitation and luciferase reporter assays, we observed the interaction of $PPAR{\gamma}$ with the miR-17 promoter. Mutagenesis experiments showed that the -677/-655 region of the miR-17 promoter could function as a PPRE site. These results suggest that $PPAR{\gamma}$ is essential for transcriptional activation of the miR-17 gene, thereby contributing to understanding the molecular mechanism of adipogenesis in adipocytes.
Keywords
Adipogenesis; microRNA; peroxisome proliferator-activated receptor ${\gamma}$; 3T3-L1 cells;
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