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http://dx.doi.org/10.5352/JLS.2014.24.3.252

Isolation, Quality Evaluation, and Seasonal Changes of Bakkenolide B in Petasites japonicus by HPLC  

Kim, Tae Hoon (Department of Horticultural Bioscience, Pusan National University)
Kim, Do Youn (Department of Horticultural Bioscience, Pusan National University)
Jung, Won Jung (Department of Horticultural Bioscience, Pusan National University)
Nagaiya, Ravichandran (Department of Horticultural Bioscience, Pusan National University)
Son, Beung Gu (Department of Horticultural Bioscience, Pusan National University)
Park, Young Hoon (Department of Horticultural Bioscience, Pusan National University)
Kang, Jum Soon (Department of Horticultural Bioscience, Pusan National University)
Lee, Young Jae (Department of Horticultural Bioscience, Pusan National University)
Im, Dong-Soon (Department of Pharmaceutics, Pusan National University)
Lee, Young-Geun (Department of Food Science and Technology, Pusan National University)
Choi, Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Choi, Young-Whan (Department of Horticultural Bioscience, Pusan National University)
Publication Information
Journal of Life Science / v.24, no.3, 2014 , pp. 252-259 More about this Journal
Abstract
The leaves of Peatasites japonicus are a traditional oriental medicine with diverse biological activities. A simple and specific analytical method for the quantitative determination of bakkenolide B constituents from methanolic extract of the leaves of P. japonicus was developed. Bakkenolide B was isolated from the leaves of P. japonicus, and its structure was elucidated based on 1D, 2D NMR, and GC-MS spectral data. A liquid chromatographic method was developed to evaluate the quality of P. japonicus through determination of major active compound, bakkenolide B. The wavelengths at 254 and 215 nm were chosen to determine bakkenolide B. The recovery of the method was in the range of 98.6 to 103.1%, and bakkenolide B showed good linearity ($r^2$=0.999) within test ranges. The developed method was applied to the determination of bakkenolide B in the plant part and seasonal changes. The results showed that the content of bakkenolide B in the leaf was higher than in the petiole and rhizome. In this study, a simple, rapid, and reliable high-performance liquid chromatography method was used to determine the percentage and composition of bakkenolide B in P. japonicus procured from different Petasites species plants in South Korea. The method can be employed in routine quantitative analysis and quality control of different products in the market.
Keywords
Bakkenolide B; HPLC; Petasites japonicus; quality evaluation; seasonal change;
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