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http://dx.doi.org/10.5352/JLS.2014.24.3.234

Isolation and Identification of a Novel Anticancer Compound from Solanum nigrum  

Yun, Hee Jung (Blue-Bio Industry RIC, Dongeui University)
Jung, Jong Hun (Department of Life Science and Biotechnology, College of Natural Science, Dongeui University)
Hyun, Sook Kyung (Blue-Bio Industry RIC, Dongeui University)
Kim, Byung Woo (Blue-Bio Industry RIC, Dongeui University)
Kwon, Hyun Ju (Blue-Bio Industry RIC, Dongeui University)
Publication Information
Journal of Life Science / v.24, no.3, 2014 , pp. 234-241 More about this Journal
Abstract
To identify and isolate anticancer active compounds from Solanum nigrum, S. nigrum was extracted with MeOH and then fractionated with various organic solvents ($CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$). The cytotoxic effects of the MeOH extracts from S. nigrum and its organic solvent-soluble fractions were also tested in HT29 cells. All the MeOH extracts of S. nigrum and its organic-solvent extracts induced cytotoxicity in the HT29 cells. Among the extracts, $H_2O$ was the most effective. The $H_2O$ extract was purified further by repeated silica gel, Sephadex LH-20, Diaion HP- 20, and RP-18 column chromatography. An active anticancer compound, Des-N-26-methylene-dihydrotomatidine, was isolated with a molecular weight of 416 and a molecular formula of $C_{28}H_{48}O_2$. Analysis of the cytotoxic effects of Des-N-26-methylene-dihydrotomatidine on the HT29 cells compared to those of tomatine and tomatidine are similar in its structure, is higher than tomatidine above the 40 ${\mu}g/ml$ concentration, but lower than tomatine. This is the first study to describe the anticancer activity of Des-N-26-methylene-dihydrotomatidin, isolated from S. nigrum. Des-N-26- methylene-dihydrotomatidine seems to have potential as a natural bioactive compound.
Keywords
Anticancer activity; anti-cancer active compound; Des-N-26-methylene-dihydrotomatidine; HT29 cells; Solanum nigrum;
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