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http://dx.doi.org/10.5352/JLS.2014.24.2.112

Effects of Rubus coreanus and Artemisia princeps Extracts on the Ultraviolet B-Induced DNA Damage Responses in HaCaT Cells  

Lee, Seok Hee (Research Institute for Basic Science and Division of Biological Science, Wonkwang University)
Ha, Se Eun (Research Institute for Basic Science and Division of Biological Science, Wonkwang University)
Lee, Jun Kyoung (Hanpoong Pharm. Co. LTD.)
Park, Jong Kun (Research Institute for Basic Science and Division of Biological Science, Wonkwang University)
Publication Information
Journal of Life Science / v.24, no.2, 2014 , pp. 112-117 More about this Journal
Abstract
We investigated the effects of extracts from Rubus coreanus (RC) and Artemisia princeps var. orientalis (AP) on DNA damage response in ultraviolet B (UVB)-exposed HaCaT cells. Cell activity upon treatment for 24 h with RC or AP alone was similar to or greater than that of the nontreated control. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, cell activity increased in a concentration-dependent manner. Nuclear fragmentation analysis showed that postincubation with RC or AP decreased UVB-induced apoptosis by about 20% and 15%, respectively, of that in cells postincubated with growth medium. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, the level of cyclobutane pyrimidine dimer decreased in a concentration- dependent manner. Western blot analysis showed that treatment of cells not exposed to UVB with RC or AP alone did not significantly change the levels of phospho-p53 and GADD45 protein. Interestingly, when UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, phospho-p53 and GADD45 levels decreased in a concentration dependent manner. Our results suggest that RC and AP extract assist the survival of UVB-exposed cells in parallel with a decrease in levels of UVB-induced DNA damage and damage-response proteins, such as p53 and GADD45.
Keywords
Artemisia princeps var. orientalis; GADD45; p53; Rubus coreanus; UVB;
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