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http://dx.doi.org/10.5352/JLS.2014.24.1.74

Fatty Acid Composition and Antiproliferative Activity of Extracts from Euphorbia Supina  

Choi, Hyang Mi (Division of Marine Environment & Bioscience, Korea Maritime and Ocean University)
Lim, Sun Young (Division of Marine Environment & Bioscience, Korea Maritime and Ocean University)
Publication Information
Journal of Life Science / v.24, no.1, 2014 , pp. 74-80 More about this Journal
Abstract
The objective of this study was to determine the fatty acid composition and the antiproliferative effect of extracts and fractions from Euphorbia supina. With regards the fatty acid composition, the percentages of 18:3n-3 in acetone/methylene chloride (A+M) and methanol (MeOH) extracts were 53.4 and 42.1%, respectively. Among the fractions, an 85% aqueous methanol (85% aq. MeOH) fraction contained the highest percentage of 18:3n-3. Treatments with crude extracts and fractions significantly inhibited the growth of HT-29 and AGS human cancer cell lines (p<0.05). The A+M extract showed a higher inhibitory effect on the growth of both cancer cells compared to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions exerted a greater inhibitory effect on the proliferation of both types of cancer cells. Our results suggest that 85% aq. MeOH and n-hexane fractions exert potent inhibitory effects on the proliferation of human cancer cells.
Keywords
Antiproliferative; Euphorbia supina; fatty acid composition; human cancer cell;
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1 Wachi, A. M., Sinclari, L. A., Wilkinson, R. G., Enser, M., Wood, J. D. and Fisher, A. V. 2002. Effect of dietary fat source and breed on the carcass composition, n-3 poly-unsaturated and conjugated linoleic acid content of sheep meat and adipose tissue. Br J Nutr 88, 697-709.   DOI   ScienceOn
2 Yu, L., Jiang, B. P., Luo, D., Shen, X. C., Guo, S., Duan, J. A. and Tang, Y. P. 2012. Bioactive components in the fruits of Ziziphusjujube Mill.Against the inflammatory irritant action of Euphorbia plants. J Ehnopharmacol 19, 239-244.
3 Cha, B. C., Kim, J. A. and Lee, Y. S. 1996. Cytotoxic activities of Panax ginseng and Euphorbia humifusa in human brain tumor cells. Korean J Pharmacogn 27, 350-353.   과학기술학회마을
4 Agata, I., Hatano, T., Nakaya, Y., Sugaya, T., Nushibe, S., Yochida, T. and Okuda, T. 1991. Tannins and related polyphenols of Euphorbiaceous plants. Eumaculin A and eusupinin A, and accompanying polyphenols from Euphorbia maculate L. and E. supine Rafin. Chem Pharm Bull 39, 881-883.   DOI
5 An, D. H., Cho, S. J., Jung, E. S., Lee, H. J. and Hwang, J. H. 2006. Antioxidant and anticancer activities of water extracts from Ceramium kondoi. J Korean Soc Food Sci Nutr 35, 1304-1308.   과학기술학회마을   DOI
6 An, R. B., Kwon, J. W., Kwon, T. O., Chung, W. T., Lee, H. S. and Kim, Y. C. 2007. Chemical constituents from the whole plants of Euphorbia supine Rafin. Korean J Pharmacogn 38, 291-295.
7 Chen, H., Wang, Z. and Yang, L. 2011. Analysis of euphornin in Euphorbia helioscopia L. and its cytotoxicity to mice lung adenocarcinoma cells. Nat Prod Res 1, 1-5.
8 Choi, J. G. 2006. The medicine of grasses, flowers and trees. pp. 193-201, Hanmunsa, Seoul.
9 Choi, M. 1991. Dietary fats and cancer. J Korean Soc Food Nutr 20, 513-518.   과학기술학회마을
10 Cognault, S., Jourdan, M. L., Germain, E., Pitavy, R., Morel, E., Durand, G., Bougnoux, P. and Lhuillery, C. 2000. Effect of an $\alpha$-linolenic acid-rich diet on rat mammary tumor growth depends in the dietary oxidative status. Nutr Cancer 36, 33-41.   DOI
11 Dommels, Y. E. M., Alink, G. M., Linssen, J. P. H. and Ommen, B. V. 2002. Effects of n-6 and n-3 polyunsaturated fatty acids on gap junctional intercellular communication during spontaneous differentiation of the human colon adenocarcinomacell line Caco-2. Nutr Cancer 42, 125-130.   DOI
12 Denizot, F. and Lang, R. 1986. Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89, 271-277.   DOI   ScienceOn
13 da Mota, M. F., Benfica, P. L., Batista, A. C., Martins, F. S., Paula, J. R. and Valadares, M. C. 2012. Investigation of Ehrlich ascites tumor cell dealth mechanisms induced by Synadanium umbellatum Pax. J Enthnopharmacol 139, 319-329.   DOI
14 Dai, J., Shen, J., Pan, W., Sehn, S. and Das, U. N. 2013. Effect of polyunsaturated fatty acids on the growth of gastric cancer cells in vitro. Lipids Health Disease 12, 71-86.   DOI
15 Fang, Z., Zeng, X., Zhang, Y. and Zhou, G. 1993. Chemical constituents of spotted leaf euphorbia (Euphorbia supina). Zhongcayao 24, 230-233.
16 Folch, J., Lees, M. and Stanley, G. S. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509.
17 Grever, M. C. B. Cancer drug discovery and development. In Cancer: Principles and Practice of Oncology. pp. 328-339, De Vita VHS, Rosenberg SA, eds. Lippinocott-Raven, Philadelphia, USA.
18 Habermann, N., Christian, B., Luckas, B., Pool-Zobel, B. L., Lund, E. K. and Glei, M. 2009. Effects of fatty acids on metabolism and cell growth of human colon cell lines of different transformation state. Int Union Biochem Molecular Biol 35, 460-467.
19 Montopoli, M., Bertin, R., Chen, Z., Bolcato., Caparrotta, L. and Froldi, G. 2012. Croton lechleri sap and isolated alkaloid taspine exhibit inhibition against human melanoma SK23 and colon cancer HT29 cell lines. J Ehnopharmacol 144, 747-753.   DOI
20 Hong, H. K., Kwak, J. H., Kang, S. C., Lee, J. W., Park, J. H., Ahn, J. W., Kang, H. S., Choung, E. S. and Zee, O. P. 2008. Antioxidative constitutents from whole plants of Euphorbia supina. Korean J Pharmacogn 39, 260-264.
21 Lee, C. B. 1989. An illusted plant book. pp. 511, Hyang- MunSa, Seoul, Korea.
22 Morrison, W. R. and Smith, L. M. Preparation of fatty acid methyl esters and dimethlyacetals from lipids with boron fluoride-methanol. J Lipid Res 5, 600-608.
23 Pascual-Villalobos, M. J. and Lopez, M. D. 2010. Leaf lipids from Euphorbia lagascae Spreng. and Euphorbia lathyris L. Ind Crops Prod 32, 560-565.   DOI
24 Rose, D. P., Connolly, J. M., Rayburn, J. and Coleman, M. 1995. Influence of diets containing eicosapentaenoic or docosahexaenoic acid on growth and metastasis of breast cancer cells in nude mice. J Natl Cancer Inst 87, 587-592.   DOI   ScienceOn
25 Salem, M., Reyer, M. and Karanian, J. 1996. Losses of arachidonic acid in rat liver after alacohol inhalation. Lipids 31, 153-156.   DOI   ScienceOn
26 Tanaka, R., Kurimoto, M., Yoneda, M. and Matsunaga S. 1990. 17$\beta$,21$\beta$-Epoxyhopan-3$\beta$-ol and $\beta$-alnincanol from Euphorbia supina. Phytochemistry 29, 2253-2256.   DOI
27 Tanaka, R. and Matsunaga, S. 1999. Terpenoids and steroids from several Euphorbiaceae and Pinaceae plants. Yakugaku Zasshi 119, 319-339.
28 Chung, B. S. and Kim, H. G. 1985. Studies on the terpenoid constituents of Euphorbia supine Rafin. Korean J Pharmacogn 16, 155-159.
29 Lee, S. H., Tanaka, T., Nonaka, G. and Nishioka, I. 1991. Tannins and related compounds. CV. Monomeric and dimeric hydrolyzable tannins having a dehydrohyxahydroxydiphenoyl group, supinanin, euphoscopin, euphorhelin and jolkianin, from Euphorbia species. Chem Pharm Bull 39, 630-638.   DOI