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http://dx.doi.org/10.5352/JLS.2014.24.1.67

Opuntia humifusa Supplementation Reduces Fat Weight by Increasing PPAR-γ and PGC-1α Protein Expression in the Skeletal Muscle of Rats  

Kwon, Daekeun (Laboratory of Sports Nutrition, Sunmoon University)
Kang, Junyong (Laboratory of Sports Nutrition, Sunmoon University)
Kim, Jaeseung (Laboratory of Sports Nutrition, Sunmoon University)
Song, Youngju (Laboratory of Sports Nutrition, Sunmoon University)
Publication Information
Journal of Life Science / v.24, no.1, 2014 , pp. 67-73 More about this Journal
Abstract
This study was conducted to investigate the effects of supplementation with Opuntia humifusa on the expression of peroxisome proliferator-activated receptor-delta (PPAR-${\delta}$), peroxisome proliferator-activated receptor-gamma (PPAR-${\gamma}$) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-$1{\alpha}$) in the skeletal muscle of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats at 6 weeks of age were randomly divided into 2 groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). The rats were fed a high-fat diet (CG) or a high-fat diet supplemented with 5% O. humifusa (EG) for 8 weeks. The results showed that the abdominal fat pad and epididymal fat pad weights were significantly lower in the EG than in the CG (p<0.01). In the blood, serum glucose, triglycerides, and total cholesterol in the EG group were lower than in the CG (p<0.01). The expression of PPAR-${\gamma}$ and PGC-$1{\alpha}$ protein in the skeletal muscle of the EG was increased compared with that of the CG (p<0.05). These results indicate that 8 weeks of O. humifusa supplementation lowers serum glucose and triglyceride levels and suppresses weight gain by reducing fat weight through an increase in the expression of PPAR-${\gamma}$ and PGC-$1{\alpha}$ in the muscle tissue of rats.
Keywords
High-fat diet; Opuntia humifusa; PPAR-${\delta}$; PPAR-${\gamma}$; PGC-$1{\alpha}$;
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