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http://dx.doi.org/10.5352/JLS.2014.24.11.1231

Probiotic Properties of Lactobacillus strains Isolated from Kimchi  

Choi, Hye Jung (Department of Biology, Changwon National University)
Lim, Bo Ram (Department of Biology, Changwon National University)
Kim, Dong Wan (Department of Microbiology, Changwon National University)
Kwon, Gi-Seok (School of Bioresource Science, Andong National University)
Joo, Woo Hong (Department of Biology, Changwon National University)
Publication Information
Journal of Life Science / v.24, no.11, 2014 , pp. 1231-1237 More about this Journal
Abstract
The objective of this study was to evaluate the safety and functional properties of four potential probiotic strains isolated from Kimchi, traditional Korean fermented vegetables. Based on being higher tolerance to bile salts and showing higher acid resistance or hydrophobic properties, one Lactobacillus arizonensis strain (BCNU 9032) and three L. brevis strains (BCNU 9037, BCNU 9098 and BCNU 9101) were selected in the screening experiment. All strains can survived up to 99% after 3h culture in pH 2.5 and resistant to 1% bile salts. These strains also showed good antimicrobial activities against a number of food borne pathogens, especially against Escherichia coli and Shigella sonnei. The ability to lower cholesterol levels of L. arizonensis BCNU 9032 and L. brevis 9037 were demonstrated by bile salt hydrolytic activity and cholesterol assimilation tests. Moreover, L. brevis BCNU 9098 and BCNU 9101 showed higher adherence to Caco-2 cells (12.76 and 11.86%, respectively) than Lactobacillus rhamnosus GG, a commercial probiotic strain used worldwide. The results suggest that these strains could be used as probiotics.
Keywords
Adhesion abilities; cholesterol assimilation; Lactobacillus strains; potential probiotics;
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1 Alander, M., Satokari, R., Korpela, R., Saxelin, M., Vilpponen-Salmela, T., Mattila-Sandholm, T. and Von Wright, A. 1999. Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl Environ Microbiol 65, 351-354.
2 Allain, C. C., Poon, L. S., Chen, C. G., Richmond, W. and Paul, C. 1974. Enzymatic determination of total serum cholesterol. Clin Chem 20, 470-475.
3 Bar-Ness, R., Avrabamy, N., Matsuyama, T. and Rosenberg, M. 1988. Increased cell surface hydrophobicity of a Serraia marcescens NS 38 mutant lacking wetting activity. J Bacteriol 170, 4361-4364.   DOI
4 Borriello, S. P., Hammes, W. P., Holzapfel, W., Marteau, P., Schrezenmeir, J., Vaara, M. and Valtonen, V. 2003. Safety of probiotics that contain lactobacilli or bifidobacteria. Clin Infect Dis 36, 775-780.   DOI   ScienceOn
5 EFSA. (2008). Technical guidance prepared by the panel on additives and products or substances in animal feed (FEEDAP) on the update of the criteria used in the assessment of bacterial resistance to antibiotics of human and veterinary importance. EFSA J 732, 1-15.
6 Cho, E. J., Rhee, S. H., Lee, S. M. and Park, K. Y. 1997. In vitro antimutagenic and anticancer effects of kimchi fractions. J Korean Ass Cancer Prev 2, 113-121.
7 Doyle, R. J. and Rosenberg. M. 1995. Measurement of microbial adhesion to hydrophobic substrates. Methods Enzymol 253, 542-550.   DOI
8 Giraffa, G., Chanishvili, N. and Widyastuti, Y. 2010. Importance of lactobacilli in food and feed biotechnology. Res Microbiol 161, 480-487.   DOI   ScienceOn
9 Hazen, K. C., Lay, J. G., Hazen, B. W., Fu, R. C. and Murthy, S. 1990. Partial biochemical charaterization of cell surface hydrophobicity and hydrophilicity of Candida albicans. Infect Immun 58, 3469-3476.
10 Bang, J. H., Shin, H, J., Choi, H. J., Kim, D. W., Ahn, C. S., Jeong ,Y. K. and Joo, W. H. 2012. Probiotic potential of Lactobacillus isolates. J Life Sci 22, 251-258.   과학기술학회마을   DOI   ScienceOn
11 Keisuke, T., Emiko, H., Nakagawa, G. U. and Masuo, S. 1996. Long-term effect of dietary fiber and fat intake on Japanese colon cancer mortality. Biosc Microflora 15, 77-74.   DOI
12 Lee, H. J., Yoon, H. S., Ji, Y. S., Kim, H, N., Park, H. J., Lee, J. E., Shin, H. K. and Holzapfel, W. H. 2011. Functional properties of Lactobacillus strains isolated from kimchi. Int J Food Microbiol 145, 155-161.   DOI
13 Kim, B. K., Park, K. Y., Kim, H. Y., Ahn, S. C. and Cho, E. J. 2011. Anti-aging effects and mechanisms of Kimchi during fermentation under stress-induced premature senescence cellular system. Food Sci Biotechnol 20, 643-649.   과학기술학회마을   DOI
14 Mathara, J. M., Schillinger, U., Guigas, C., Franz, C. M. A. P., Kutima, P. M., Mbugua, S., Shin, H. K. and Holzapfel, W. H. 2008. Functional characteristics of Lactobacillus spp. Form traditional Maasai fermented milk products in Kenya. Int J Food Microbiol 126, 57-64.   DOI   ScienceOn
15 Kim, H. J., Kwon, M. J., Seo, J. M., Kim, J. K., Song, S. H., Suh, H. S. and Song, Y. O. 2004. The effect of 3-(4'-hydroxyl-3',5'-dimethoxyphenyl) propionic acid in Chinese cabbage kimchi on lowering hypercholesterolemia. J Korean Soc Food Sci Nutr 33, 52-58.   과학기술학회마을   DOI
16 Maragkoudakisa, P. A., Zoumpopouloua, G., Miarisa, C., Kalantzopoulosa, G., Potb, B. and Tsakalidoua, E. 2006. Probiotic potential of Lactobacillus strains isolated from dairy droducts. Int Dairy J 16, 189-199.   DOI   ScienceOn
17 Mishra, V. and Prasad, D. N. 2005. Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int J Food Microbiol 103, 109-115.   DOI   ScienceOn
18 Ramos, C. L., Thorsen, L., Schwan, R. F. and Jespersen, L. 2013. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products. Food Microbiol 36, 22-29.   DOI
19 Saito, N. and Nei, M. 1987. The neighbor-joining method, a new method for reconstructing phylogenetic trees. Mol Biol Evol 79, 426-434.
20 SCAN. 2002. Opinion of the scientific committee on animal nutrition on the criteria for assessing the safety of micro-organisms resistant to antibiotics of human clinical and veterinary importance. European commission, health and consumer protection directorate general; directorate C, scientific opinions, 18 April 2002.
21 Thirabunyanon, M., Boonprasom, P. and Niamsup, P. 2009. Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells. Biotechnol Lett 31, 571-576.   DOI   ScienceOn
22 Sheo, H. J. and Seo, Y. S. 2003. The antibacterial action of Chinese cabbage kimchi juice on Staphylococcus aureus, Salmonellla enteritidis and Enterobacter cloacae. J Korean Soc Food Sci Nutr 32, 1351-1356.   DOI
23 Shin, K., Chae, O., Park, I., Hong, S. and Choe, T. 1998. Antitumor effects of mice fed with cell lysates of Lactobacillus plantarum isolated from kimchi. Korean Soc Biotechnol Bioeng J 13, 357-363.
24 Sneath, P. H. A. 1986. Endospore-forming gram-positive rods and cocci, pp. 1104-1139. In: Sneath, P. H. A., Mair, N. S., Sharpe, M. E. and Holt, J. G. (eds.), Bergey's manual of systematic Bacteriology. Williams & Wilkins, Baltimore, Maryland, USA.
25 Todorv, S. D. and Dicks, L. M. T. 2008. Evaluation of lactic acid bacteria from kefir, molasses and olive brine as possible probiotics based on physiological properties. Ann Microbiol 58, 661-670.   DOI
26 Vrese, M., Stegelmann, A., Ritcher, B., Fenselau, S., Laue, C. and Schrezenmeir, J. 2001. Probiotics: compensation for lactase insufficiency. Am J Clin Nutr 73, S421-S429.   DOI
27 Danielsen, M. and Wind, A. A. 2003. Susceptibility of Lactobacillus spp. to antimicrobial agents. Int J Food Microbiol 82, 1-11.   DOI   ScienceOn