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http://dx.doi.org/10.5352/JLS.2014.24.11.1224

Antiadipogenic Effects of Red Radish (Raphanus sativus L.) Sprout Extract in 3T3-L1 Preadipocytes  

Kim, Da Hye (Jeonju Biomaterials Institute)
Kim, Sang Jun (Jeonju Biomaterials Institute)
Jeong, Seung-Il (Jeonju Biomaterials Institute)
Cheon, Chun Jin (Aenong Association)
Kim, Seon-Young (Jeonju Biomaterials Institute)
Publication Information
Journal of Life Science / v.24, no.11, 2014 , pp. 1224-1230 More about this Journal
Abstract
The red radish (Raphanus sativus L.; RR) sprout is a plant of the cruciferous family. In this study, we elucidated the effect of the water extract of RR sprout (RRSE) against ${\alpha}$-amylase, ${\alpha}$-glucosidase, and pancreatic lipase enzyme activity and adipogenesis in 3T3-L1 preadipocytes. ${\alpha}$-amylase, ${\alpha}$-glucosidase, and pancreatic lipase enzyme activity was inhibited in a concentration-dependent manner by RRSE treatment. RSSE also abolished adipocyte differentiation and lipid and triglyceride accumulation without cytotoxicity in 3T3-L1 adipocytes. In addition, RRSE modulated the expression of the proteins related to adipogenic transcription factors: peroxisome proliferator-activated receptor (PPAR)${\gamma}$, sterol regulatory element-binding protein 1 (SREBP-1), and CCAT/enhancer binding protein (C/EBP)${\alpha}$. RRSE also suppressed expression of the proteins responsible for lipid synthesis, transport, and storage: adiponectin, fatty acid synthesis (FAS), perilipin, and fatty acid bind protein-4 (FABP4). This study showed that RRS treatment has the potential to inhibit obesity by controlling the expression of adipogenic transcription factors and adipogenic proteins.
Keywords
Adipogenesis; lipase activity; lipid accumulation; obesity; red radish sprout;
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