Browse > Article
http://dx.doi.org/10.5352/JLS.2014.24.11.1187

Analysis of Genes Expressed during Pepper-Phytophthora capsici Interaction using EST Technology  

Kim, Dongyoung (Department of Biomaterial Control, Dongeui University)
Lee, Jong-Hwan (Department of Biomaterial Control, Dongeui University)
Choi, Woobong (Department of Biomaterial Control, Dongeui University)
Publication Information
Journal of Life Science / v.24, no.11, 2014 , pp. 1187-1192 More about this Journal
Abstract
Pepper, consumed as a typical spice food around world, is mainly cultivated in warm countries, including Korea, China, and Mexico. Phytophthora capsici is a pathogen on several economically important crops, including pepper. The oomycete attacks the roots, stems, leaves, and fruit of the host plants. To understand the molecular mechanisms underlying development of the disease, the genes expressed during pepper-P. capsici interaction were explored by analyzing expressed sequence tags (ESTs). A cDNA library was constructed from total RNA extracted from pepper leaves challenged with P. capsici for three days, resulting in an early stage of symptom development for comparable interaction. A comprehensive analysis of single-pass sequencing of 5,760 randomly selected cDNA clones extracted 5,148 high-quality entries for contig assembly, which generated 2,990 unigenes. A homology search of the unigenes with BLASTX resulted in 2,409 matches, of which 606 showed classified functional catalogs.
Keywords
Expressed sequence tag (EST); pepper; Phytophthora capsici;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Williamson, V. M. and Gleason, C. A. 2003. Plant-nematode interactions. Curr Opin Plant Biol 6, 1-7.   DOI
2 Yamamoto, K. and Sasaki, T. 1997. Large scale EST sequencing in rice. Plant Mol Biol 35, 135-144.   DOI   ScienceOn
3 Gyorgyey, J., Vaubert, D., Jimenez-Zurdo, J. I., Charon, C., Troussard, L., Kondorosi, A. and Kondorosi, E. 2000. Analysis of Medicago truncatula nodule expressed sequence tags. Mol Plant Microbe Interact 13, 62-71.   DOI
4 Hong, J. K., Jung, H. W., Kim, Y. J. and Hwang, B. K. 2000. Pepper gene encoding a basic class II chitinase is inducible by pathogen and ethephon. Plant Sci 159, 39-49.   DOI   ScienceOn
5 Hwang, B. K. 2001. Cytology, physiology and molecular genetics of resistance to Phytophthora blight in pepper plants. Plant Pathol J 17, 9-21.
6 Hwang, B. K. and Kim, C. H. 1995. Phytophthora blight of pepper and its control in Korea. Plant Dis 79, 221-227.   DOI
7 Jongedijk, E., Tigelaar, H., Van Roekel, J. S. C., Bres- Vloemans, S. A., Dekker, I., Van Den Elzen, P. J. M., Cornelissen, B. J. C. and Melchers, L. S. 1995. Synergistic activity of chitinases and beta-1,3-glucanases enhances fungal resistance in transgenic tomato plants. Euphytica 85, 173-180.   DOI
8 Jung, H. W. and Hwang, B. K. 2000. Pepper gene encoding a basic beta-1,3-glucanase is differentially expressed in pepper tissues upon pathogen infection and ethephon or methyl jasmonate treatment. Plant Sci 156, 23-34.   DOI   ScienceOn
9 Keon, J., Bailey, A. and Hargreaves, J. 2000. A group of expressed cDNA sequences from the wheat fungal leaf blotch pathogen, Mycosphaerella graminicola (Septoria tritici). Fungal Genet Biol 29, 118-133.   DOI   ScienceOn
10 Jung, H. W., Kim, W. and Hwang, B. K. 2003. Three pathogen- induced genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, and environmental stresses. Plant Cell Environ 26, 915-928.   DOI   ScienceOn
11 Kim, S., Ahn, I. P. and Lee, Y. H. 2001. Analysis of genes expressed during rice-Magnaporthe grisea interactions. Mol Plant Microbe In 14, 1340-1346.   DOI   ScienceOn
12 Kamoun, S., Hraber, P., Sobral, B., Nuss, D. and Govers, F. 1999. Initial assessment of gene diversity for the Oomycete pathogen Phytophthora infestans based on expressed sequences. Fungal Genet Biol 28, 94-106.   DOI   ScienceOn
13 Karlsson, M., Olson, A. and Stenlid, J. 2003. Expressed sequences from the basidiomycetous tree pathogen Heterobasidion annosum during early infection of scots pine. Fungal Genet Biol 39, 51-59.   DOI
14 Kim, J. B., Lee, S. G., Ha, S. H., Lee, M. C., Ye, W. H., Lee, J. Y., Lee, S. W., Kim, J. B., Cho, K. J. and Hwang, Y. S. 2001. Molecular cloning and characterization of sesquiterpene cyclase cDNAs from pepper plant infected with Phytophthora capsici. Agric Chem Biotechnol 44, 59-64.
15 Kruger, W. M., Pritsch, C., Chao, S. and Muehlbauer, G. J. 2002. Functional and comparative bioinformatic analysis of expressed genes from wheat spikes infected with Fusarium graminearum. Mol Plant Microbe Interact 15, 421-427.   DOI   ScienceOn
16 Lee, S. C., Hong, J. K., Kim, Y. J. and Hwang, B. K. 2000. Pepper gene encoding thionin is differentially induced by pathogens, ethylene and methyl jasmonate. Physiol Mol Plant Pathol 56, 207-216.   DOI   ScienceOn
17 Qutob, D., Hraber, P. T., Sobral, B. W. S. and Gijzen, M. 2000. Comparative analysis of expressed sequences in Phytophthora sojae. Plant Physiol 123, 243-253.   DOI   ScienceOn
18 Bowers, J. H. and Mitchell, D. J. 1991. Relationship between inoculum level of Phytophthora capsici and mortality of peppers. Phytopathology 81, 178-184.   DOI
19 Adams, M. D., Kelley, J. M., Gocayne, J. D., Dubnick, M., Polymeropoulos, M. H., Xiao, H., Merril, C. R., Wu, A., Olde, B. and Moreno, R. F. et al. 1991. Complementary DNA sequencing: expressed sequence tags and human genome project. Science 252, 1651-1656.   DOI   ScienceOn
20 Black, L. L., Green, S. K., Hartman, G. L. and Poulos, J. M. 1991. Pepper diseases: a field guide. Asian vegetable research and development center, Tainan, Taiwan. AVRDC Publication 91, 347.
21 Ha, S. H., Kim, J. B., Hwang, Y. S. and Lee, S. W. 2003. Molecular characterization of three 3-hydroxy-3-methylglutaryl-CoA reductase genes including pathogen-induced Hmg2 from pepper (Capsicum annuum). Biochim Biophys Acta 1625, 253-260.   DOI
22 Egea, C., Dickinson, M. J., Candela, M. and Candela, M. E. 1999. Beta-1,3-glucanase isoenzymes and genes in resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici. Physiol Plantarum 107, 312-318.   DOI
23 Erwin, D. C. and Rebeiro, O. K. 1996. Phytophthora, Diseases Worldwide. APS Press, St. Paul. 562 pp.
24 Rauyaree, P., Choi, W., Fang, E., Blackmon, B. and Dean, R. A. 2001. Genes expressed during early stages of rice infection with the rice blast fungus Magnaporthe grisea. Mol Plant Pathol 2, 347-354.   DOI   ScienceOn
25 Lee, Y. K., Hong, J. K., Hippe-Sanwald, S. and Hwang, B. K. 2000. Histological and ultrastructural comparison of compatible, incompatible and DL-b-amino-n-butyric acid-induced resistance responses of pepper stems to Phytophthora capsici. Physiol Mol Plant Pathol 57, 269-280.   DOI   ScienceOn
26 Lee, Y. K., Hippe-Sanwald, S., Jung, H. W., Hong, J. K., Hause, B. and Hwang, B. K. 2000. In situ localization of chitinase mRNA and protein in compatible and incompatible interactions of pepper stems with Phytophthora capsici. Physiol Mol Plant Pathol 57, 111-121.   DOI   ScienceOn
27 Pritsch, C., Vance, C. P., Bushnell, W. R., Somers, D. A., Hohn, T. M. and Muehlbauer, G. J. 2001. Systemic expression of defense response genes in wheat spikes as a response to Fusarium graminearum infection. Physiol Mol Plant Pathol 58, 1-12.   DOI
28 Soanes, D. M., Skinner, W., Keon, J., Hargreaves, J. and Talbot, N. J. 2002. Genomics of phytopathogenic fungi and the development of bioinformatic resources. Mol Plant Microbe Interact 15, 421-427.   DOI   ScienceOn
29 Thomas, S. W., Rasmussen, S. W., Glaring, M. A., Rouster, J. A., Christiansen, S. K. and Oliver, R. P. 2001. Gene identification in the obligate fungal pathogen Blumeria graminis by expressed sequence tag analysis. Fungal Genet Biol 33, 195-211.   DOI   ScienceOn
30 White, J. A., Todd, J., Newman, T., Focks, N., Girke, T., de Ilarduya, O. M., Jaworski, J. G., Ohlrogge, J. B. and Benning, C. 2000. A new set of Arabidopsis expressed sequence tags from developing seeds. The metabolic pathway from carbohydrates to seed oil. Plant Physiol 124, 1582-1594.   DOI   ScienceOn