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http://dx.doi.org/10.5352/JLS.2014.24.11.1174

Genetic Variation in Mutants Induced by Gamma Ray in Hypsizigus marmoreus  

Kim, Jong-Bong (Department of Medical Life Science, Catholic University of Daegu)
Yu, Dong-Won (Department of Medical Life Science, Catholic University of Daegu)
Publication Information
Journal of Life Science / v.24, no.11, 2014 , pp. 1174-1179 More about this Journal
Abstract
This research was carried out to evaluate whether gamma ray is a useful tool for breeding new strains of mushrooms. For this research, 5 mutant groups, 20 strains of Hypsizigus marmoreus, 2 strains of Lyophyllum decastes, and 1 strain of Lyophyllum shimeji were used. Monokaryon spores from one variety of H. marmoreus were irradiated with 50~2,000 Gy of gamma ray. The propriety dose was 50~200 Gy for mutagenesis. Mutant monokaryon mycelia crossed each order to become dikaryon mycelia. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR, and the products were sequenced. The sequences of the ITS regions (16 partial rDNA, complete ITS1, 5.8 rDNA and partial rDNA) were analyzed by PCR, and strains of H. marmoreus, L. decastes, and L. shimeji were auto-sequenced. The lengths of the sequenced ITSs were 1,052~1,143 nucleotides. Genetic matrices were calculated using Nei-Li's genetic distance coefficient based on ITS sequence. The dissimilarities were 0~3.35% in strains of H. Hypsizigus. In addition, a phylogenetic tree was constructed based on ITS sequences using the neighbor-joining (NJ) method. The phylogenetic tree revealed that 23 strains and 5 mutant groups were divided into 12 clusters; the mutant groups fell into different clusters. These results show that mushroom spores were mutated effectively by gamma ray; therefore, gamma ray could be a useful tool for breeding new strains of mushrooms.
Keywords
Hypizigus marmoreus; ITS sequence; mutagenesis gama ray;
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1 Matsuzawa, T., Sano, M., Tomita I., Saitoh, J. and Ikekawa, T. 1997. Studies on the antioxidant effect of Hypsizygus marmoreus. I. effects of Hypsizygus marmoreus for antioxidant activities of mice plasma. Yakugaku Zasshi 188, 623-628.
2 Kim, J. K., Moon, D. H., Seo, G. S. and Kang, H. W. 2011. Mutagenesis of of Hypsizygus marmoreus by gamma ray irradiation. Korean J Mycol 39, 231-234   과학기술학회마을   DOI
3 Lee, S. M. 1988. A rapid high yield mini-prep method for isolation of total genomic DNA from fungi. Fungal Genet Newsl 35, 23-24.
4 Lim, Y. J., Lee, C. Y., Park J. E., Kim, S. W., Lee, H. S. and Ro, H. S. 2010. Molecular genetic classification of Hypsizigus marmoreus and development of strain-specific DNA markers. Korean J Mycol 38, 34-39.   과학기술학회마을   DOI   ScienceOn
5 Matsuzawa, T., Saitoh, H., Sano, M., Tomita, I., Ohkawa, M. and Ikekawa, T. 1998. Studies on antioxidant effect of Hypsizygus marmoreus. II. effects of Hypsizygus marmoreus for antioxidant activities of tumor-bearing mice. Yakugaku Zasshi 188, 476-481.
6 Mori, K., Kobayashi, C., Tomita, T., Inatomi, S. and Ikeda, M. 2008. Antiatherosclerotic effect of the edible mushroom Pleurotus eryngii, Grifola frondosa, and Hypsizygus marmoreus in apolipoprotein E-deficient mice. Nutr Res 28, 335-342   DOI   ScienceOn
7 Nei, M. 1972. Genetic distance between populations. Am Natur 106, 283-292.   DOI   ScienceOn
8 Ohashi, H. 2010. Trends of mushroom production and marketing, pp.18. In: Ohashi, H. Annual report of mushroom 201" Plant's world Co., Ltd.: Tokyo, Japan.
9 Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. J Evolution 39, 783-791.   DOI   ScienceOn
10 Farris, J. S., Albert, V. A., Kallersjo, M., Lipscomb, D. and Kluge, A. G. 1996. Parsimony jackknifing outperforms neighbor-joining. Cladistics 12, 99-124.   DOI   ScienceOn
11 Hirashi, A. 1992. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210-213.   DOI   ScienceOn
12 Jeong, H. Y. and Kim, K. R. 2010. Strain Improvement based on Ion beam-induced mutagenesis. Korean J Microbiol Biotechnol 38, 235-243   과학기술학회마을
13 White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322, Academic Press Inc.: Sandiego, California.
14 Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughers, P., Dodd, C., Connell, C. R., Heiner, C., Kent, S. B. and Hood, L. E. 1986. Fluorescence detection in automated DNA sequence analysis. Nature 321, 674-679.   DOI   ScienceOn
15 Swofford, D. L. 2001. PAUP-Phylog enetic analysis using parsimony (and other methods) Ver.4.0b2. Academic Press, Sunderland, MA.
16 Sneath, P. H. A. and Sokal, R. R. 1973. Numerical taxonomy, pp. 573, W. H. Freeman: San Francisco.
17 Zanabaatar, B., Kang, M. G., Seo, G. S., Lee, Y. W. and Lee, J. S. 2012. Analysis of ntritional caracteristics and pysiological fnctionality of Hypsizhgus marmoreus (Brown cultivar). Korean J Mycol 40, 104-108   DOI   ScienceOn