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http://dx.doi.org/10.5352/JLS.2013.23.9.1140

Protective Effect of Dried Mackerel Extract on Lipopolysaccharide-induced Inflammation  

Kim, Kwang-Hyuk (Department Microbiology, Kosin University College of Medicine)
Choi, Myoung Won (Department Microbiology, Kosin University College of Medicine)
Choi, Hyang Mi (Division of Marine Environment & Bioscience, Korea Maritime and Ocean University)
Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime and Ocean University)
Publication Information
Journal of Life Science / v.23, no.9, 2013 , pp. 1140-1146 More about this Journal
Abstract
The effect of dried mackerel extract on the production of nitric oxide (NO) and cytokines, including interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and interferon-${\gamma}$ (IFN-${\gamma}$), was investigated. All extracts and fractions from dried mackerel significantly reduced NO production induced by lipopolysaccharide (LPS). Among the extracts, acetone+methylene chloride (A+M), n-hexane, and 85% aqueous methanol (MeOH) showed the strongest inhibitory effects. The 85% aqueous MeOH fraction at a concentration of $10{\mu}g$ significantly decreased LPS-induced IL-6 and TNF-${\alpha}$ production after 6 hr of incubation. In the case of LPS-induced IFN-${\gamma}$ production, the 85% aqueous MeOH fraction decreased the production of IFN-${\gamma}$ afer 6, 24, and 72 hr of incubation in a dose-dependent manner. The results show that an 85% aqueous MeOH fraction inhibits the production of NO and proinflammatory cytokines (IL-6, TNF-${\alpha}$, IFN-${\gamma}$), suggesting that this fraction acts as a potent immunomodulator.
Keywords
Interferon-${\gamma}$; interleukin-6; mackerel extract; nitric oxide; tumor necrosis factor-${\alpha}$;
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