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http://dx.doi.org/10.5352/JLS.2013.23.9.1133

Specific Detection of Serratia marcescens Based on a PCR Assay and Antimicrobial Susceptibility of S. marcescens Isolated from Boar Semen  

Jung, Ji-A (Bacteriology Division, Animal and Plant Quarantine Agency)
Kim, Aeran (Bacteriology Division, Animal and Plant Quarantine Agency)
Seo, Byoung Joo (Bacteriology Division, Animal and Plant Quarantine Agency)
Jung, Suk Chan (Bacteriology Division, Animal and Plant Quarantine Agency)
Kim, In Cheul (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Chung, Ki Hwa (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Jung, Byeong Yeal (Bacteriology Division, Animal and Plant Quarantine Agency)
Publication Information
Journal of Life Science / v.23, no.9, 2013 , pp. 1133-1139 More about this Journal
Abstract
During the collection of boar semen, bacterial contamination usually occurs. The contamination has deleterious effects both on semen quality and on sow fertility. The majority of contaminants are gram-negative bacteria, especially Serratia marcescens. In this study, we developed a PCR assay for the identification of S. marcescens targeting the luxS gene (GenBank no. EF164926). S. marcescens yielded a specific 306 bp PCR product. However, no amplification was observed in the other strains tested. The detection limit of PCR was $50pg/{\mu}l$ of template DNA of S. marcescens. The antimicrobial susceptibility patterns of S. marcescens isolated from boar semen were tested using the disk diffusion method. Gentamicin, ceftiofur, florfenicol, and neomycin showed high sensitivity in this test. The minimum inhibitory concentration (MIC) was also determined by the broth microdilution method. The $MIC_{90}$ values of ceftiofur, enrofloxacin, gentamicin, and neomycin were 8, 8, 8, and $16{\mu}g/ml$, respectively. These results indicate that PCR amplification of the luxS gene is a reliable and effective method for the identification of S. marcescens and that ceftiofur, enrofloxacin, gentamicin, and neomycin are effective semen extenders for controlling S. marcescens.
Keywords
Serratia marcescens; boar semen; PCR assay; luxS gene; antimicrobials;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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