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http://dx.doi.org/10.5352/JLS.2013.23.5.609

An Investigation of Glyceollin I's Inhibitory Effect on The Mammalian Adenylyl  

Kim, Dong-Chan (Department of Biomedical Laboratory Science, Gimcheon University)
Kim, Nam Doo (Daegu-Gyeongbuk Medical Innovation Foundation, New Drug Development Center)
Kim, Sung In (Department of Biomedical Laboratory Science, Gimcheon University)
Jang, Chul-Soo (Department of Biomedical Laboratory Science, Gimcheon University)
Kweon, Chang Oh (Department of Biomedical Laboratory Science, Gimcheon University)
Kim, Byung Weon (Department of Biomedical Laboratory Science, Gimcheon University)
Ryu, Jae-Ki (Department of Biomedical Laboratory Science, Gimcheon University)
Kim, Hyun-Kyung (Department of Biomedical Laboratory Science, Gimcheon University)
Lee, Suk Jun (Department of Biomedical Laboratory Science, Gimcheon University)
Lee, Seungho (Department of Oriental Rehabilitation Medicine, Gimcheon University)
Kim, Dongjin (School of Civil and Environmental Engineering, Georgia Institute of Technology)
Publication Information
Journal of Life Science / v.23, no.5, 2013 , pp. 609-615 More about this Journal
Abstract
Glyceollin I has gained attention as a useful therapy for various dermatological diseases. However, the binding property of glyceollin I to the mammalian adenylyl cyclase (hereafter mAC), a critical target enzyme for the down-regulation of skin melanogenesis, has not been fully explored. To clarify the action mechanism between glyceollin I and mAC, we first investigated the molecular docking property of glyceollin I to mAC and compared with that of SQ22,536, a well-known mAC inhibitor, to mAC. Glyceollin I showed superiority by forming three hydrogen bonds with Asp 1018, Trp 1020, and Asn 1025, which exist in the catalytic site of mAC. However, SQ22,536 formed only two hydrogen bonds with Asp 1018 and Asn 1025. Secondly, we confirmed that glyceollin I effectively inhibits the formation of forskolin-induced cAMP and the phosphorylation of PKA from a cell-based assay. Long term treatment with glyceollin I had little effect on the cell viability. The findings of the present study also suggest that glyceollin I may be extended to be used as an effective inhibitor of hyperpigmentation.
Keywords
Glyceollin I; mammalian adenylyl cyclase; SQ22536; melanogenesis; computation modeling;
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1 Kim, H. J., Lim, J. S., Kim, W. K. and Kim, J. S. 2012. Soyabean glyceollins: biological effects and relevance to human health. Proc Nutr Soc 71, 166-174.   DOI   ScienceOn
2 Prezioso, J. A., Hearing, V. J., Muller, J., Urabe, K., Wang, N. and Gorelik, E. 1995. Impairment of the melanogenic pathway in B16 melanoma cells transfected with class I H-2 genes. Melanoma Res 5, 15-25.
3 Yoon, W. J., Kim, M. J., Moon, J. Y., Kang, H. J., Kim, G. O., Lee, N. H. and Hyun, C. G. 2010. Effect of palmitoleic acid on melanogenic protein expression in murine b16 melanoma. J Oleo Sci 59, 315-319.   DOI
4 Boue, S. M., Carter, C. H., Ehrlich, K. C. and Cleveland, T. E. 2000. Induction of the soybean phytoalexins coumestrol and glyceollin by Aspergillus. J Agric Food Chem 48, 2167-2172.   DOI   ScienceOn
5 Brown, B. L., Albano, J. D., Ekins, R. P. and Sgherzi, A. M. 1971. A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochem J 121, 561-562.
6 Busca, R. and Ballotti, R. 2000. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res 13, 60-69.   DOI   ScienceOn
7 Cheli, Y., Luciani, F., Khaled, M., Beuret, L., Bille, K., Gounon, P., Ortonne, J. P., Bertolotto, C. and Ballotti, R. 2009. alpha-MSH and Cyclic AMP elevating agents control melanosome pH through a protein kinase A-independent mechanism. J Biol Chem 284, 18699-18706.   DOI   ScienceOn
8 Daniel, O., Meier, M. S., Schlatter, J. and Frischknecht, P. 1999. Selected phenolic compounds in cultivated plants: ecologic functions, health implications, and modulation by pesticides. Environ Health Perspect 107 Suppl 1, 109-114.   DOI
9 Dwivedi, Y. and Pandey, G. N. 2008. Adenylyl cyclase- cyclicAMP signaling in mood disorders: role of the crucial phosphorylating enzyme protein kinase A. Neuropsychiatr Dis Treat 4, 161-176.
10 Gillbro, J. M. and Olsson, M. J. 2011. The melanogenesis and mechanisms of skin-lightening agents-existing and new approaches. Int J Cosmet Sci 33, 210-221.   DOI   ScienceOn
11 Graham, T. L. and Graham, M. Y. 1996. Signaling in soybean phenylpropanoid responses (Dissection of Primary, Secondary, and Conditioning Effects of Light, Wounding, and Elicitor Treatments). Plant Physiol 110, 1123-1133.
12 Han, Y. K., Park, Y. J., Ha, Y. M., Park, D., Lee, J. Y., Lee, N., Yoon, J. H., Moon, H. R. and Chung, H. Y. 2012. Characterization of a novel tyrosinase inhibitor, (2RS,4R)-2-(2,4-dihydroxyphenyl)thiazolidine-4-carboxylic acid (MHY384). Biochim Biophys Acta 1820, 542-549.   DOI   ScienceOn
13 Kim, D. C., Rho, S. H., Shin, J. C., Park, H. H. and Kim, D. 2011. Inhibition of melanogenesis by 5,7-dihydroxyflavone (chrysin) via blocking adenylyl cyclase activity. Biochem Biophys Res Commun 411, 121-125.   DOI   ScienceOn
14 Iwatsubo, K., Tsunematsu, T. and Ishikawa, Y. 2003. Isoform-specific regulation of adenylyl cyclase: a potential target in future pharmacotherapy. Expert Opin Ther Targets 7, 441-451.   DOI   ScienceOn