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http://dx.doi.org/10.5352/JLS.2013.23.3.406

Bacterial Community Structure Shift Driven by Salinity: Analysis of DGGE Band Patterns from Freshwater to Seawater of Hyeongsan River, Korea  

Beck, Bo Ram (East Sea Rim Marine and Fisheries Research Institute, Handong Global University)
Holzapfel, Wilhelm (East Sea Rim Marine and Fisheries Research Institute, Handong Global University)
Hwang, Cher Won (Global Leadership School, Handong Global University)
Do, Hyung Ki (East Sea Rim Marine and Fisheries Research Institute, Handong Global University)
Publication Information
Journal of Life Science / v.23, no.3, 2013 , pp. 406-414 More about this Journal
Abstract
The influence of a gradual increase in salinity on the diversity of aquatic bacterial in rivers was demonstrated. The denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community shift downstream in the Hyeongsan River until it joins the open ocean. Four water samples were taken from the river showing the salinity gradients of 0.02%, 1.48%, 2.63%, and 3.62%. The samples were collected from four arbitrary stations in 2.91 km intervals on average, and a DGGE analysis was performed. Based on the results of this analysis, phylogenetic similarity identification, tree analysis, and a comparison of each station were performed. The results strongly suggested that the response of the bacterial community response was concomitant to gradual changes in salinity, which implies that salt concentration is a major factor in shifting the microbiota in aquatic habitats. The results also imply a huge diversity in a relatively small area upstream from the river mouth, compared to that in open oceans or coastal regions. Therefore, areas downstream towards a river mouth or delta are could be good starting points in the search for new bacterial species and strains ("biotypes").
Keywords
Salinity; bacteria; bacterial community shift; microbial diversity;
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