Browse > Article
http://dx.doi.org/10.5352/JLS.2013.23.3.355

Withaferin A Inhibits PMA-Induced MMP-9 Expression in Human Cervical Carcinoma Caski Cells  

Kim, Dong Eun (Department of Otolaryngology, School of Medicine, Keimyung University)
Publication Information
Journal of Life Science / v.23, no.3, 2013 , pp. 355-360 More about this Journal
Abstract
Withaferin A is an active component of Withania somnifera, and has anti-inflammatory, anti-tumor, and immune modulatory effects. However, the effects of withaferin A on metalloproteinase (MMP)-9 expression and activity have not been investigated. In this study, we investigated the ability of withaferin A to inhibit MMP-9 expression and activity in PMA-treated human cervical carcinoma Caski cells. Withaferin A markedly inhibited the PMA-induced MMP-9 activity in a dose-dependent manner. Withaferin A decreased not only PMA-induced MMP-9 promoter activity but also PMA-mediated MMP-9 mRNA and protein expression in Caski cells. NF-${\kappa}B$ promoter activity, which is important in MMP-9 expression, was also decreased in combined treatment with withaferin A and PMA. Furthermore, withaferin A markedly suppressed the ability of PMA-mediated migration in Caski cells. Our findings suggest that withaferin A might inhibit PMA-induced migration through the down-regulation of MMP-9 expression and activity.
Keywords
Caski cells; matrix metalloproteinase-9; migration; NF-${\kappa}B$; withaferin A;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lee, E. J., Kim, S. Y., Hyun, J. W., Min, S. W., Kim, D. H. and Kim, H. S. 2010. Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression. Chem Biol Interact 185, 18-24.   DOI   ScienceOn
2 Lee, T. J., Um, H. J., Min do, S., Park, J. W., Choi, K. S. and Kwon, T. K. 2009. Withaferin A sensitizes TRAIL-induced apoptosis through reactive oxygen species-mediated up-regulation of death receptor 5 and down-regulation of c-FLIP. Free Radic Biol Med 46, 1639-1649.   DOI   ScienceOn
3 Festuccia, C., Giunciuglio, D., Guerra, F., Villanova, I., Angelucci, A., Manduca, P., Teti, A., Albini, A. and Bologna, M. 1999. Osteoblasts modulate secretion of urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) in human prostate cancer cells promoting migration and matrigel invasion. Oncol Res 11, 17-31.
4 Hah, N. and Lee, S. T. 2003. An absolute role of the PKC-dependent NF-kappaB activation for induction of MMP-9 in hepatocellular carcinoma cells. Biochem Biophys Res Commun 305, 428-433.   DOI   ScienceOn
5 Hung, W. C., Tseng, W. L., Shiea, J. and Chang, H. C. 2010. Skp2 overexpression increases the expression of MMP-2 and MMP-9 and invasion of lung cancer cells. Cancer Lett 288, 156-161.   DOI   ScienceOn
6 Koduru, S., Kumar, R., Srinivasan, S., Evers, M. B. and Damodaran, C. 2010. Notch-1 inhibition by Withaferin-A: a therapeutic target against colon carcinogenesis. Mol Cancer Ther 9, 202-210.
7 Kumar, B., Koul, S., Petersen, J., Khandrika, L., Hwa, J. S., Meacham, R. B., Wilson, S. and Koul, H. K. 2010. p38 mitogen- activated protein kinase-driven MAPKAPK2 regulates invasion of bladder cancer by modulation of MMP-2 and MMP-9 activity. Cancer Res 70, 832-841.   DOI   ScienceOn
8 Mohan, R., Hammers, H. J., Bargagna-Mohan, P., Zhan, X. H., Herbstritt, C. J., Ruiz, A., Zhang, L., Hanson, A. D., Conner, B. P., Rougas, J. and Pribluda, V. S. 2004. Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 7, 115-122.   DOI   ScienceOn
9 Nalla, A. K., Gorantla, B., Gondi, C. S., Lakka, S. S. and Rao, J. S. 2010. Targeting MMP-9, uPAR, and cathepsin B inhibits invasion, migration and activates apoptosis in prostate cancer cells. Cancer Gene Ther 17, 599-613.   DOI   ScienceOn
10 Ndlovu, M. N., Van Lint, C., Van Wesemael, K., Callebert, P., Chalbos, D., Haegeman, G. and Vanden Berghe, W. 2009. Hyperactivated NF-{kappa}B and AP-1 transcription factors promote highly accessible chromatin and constitutive transcription across the interleukin-6 gene promoter in metastatic breast cancer cells. Mol Cell Biol 29, 5488-5504.   DOI   ScienceOn
11 Yang, E. S., Choi, M. J., Kim, J. H., Choi, K. S. and Kwon, T. K. 2011. Withaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition. Chem Biol Interact 190, 9-15.   DOI   ScienceOn
12 Um, H. J., Min, K. J., Kim, D. E. and Kwon, T. K. 2012. Withaferin A inhibits JAK/STAT3 signaling and induces apoptosis of human renal carcinoma Caki cells. Biochem Biophys Res Commun 427, 24-29.   DOI   ScienceOn
13 Visse, R. and Nagase, H. 2003. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res 92, 827-839.   DOI   ScienceOn
14 Woo, J. H., Lim, J. H., Kim, Y. H., Suh, S. I., Min, D. S., Chang, J. S., Lee, Y. H., Park, J. W. and Kwon, T. K. 2004. Resveratrol inhibits phorbol myristate acetate-induced matrix metalloproteinase-9 expression by inhibiting JNK and PKC delta signal transduction. Oncogene 23, 1845-1853.   DOI   ScienceOn
15 Masson, V., de la Ballina, L. R., Munaut, C., Wielockx, B., Jost, M., Maillard, C., Blacher, S., Bajou, K., Itoh, T., Itohara, S., Werb, Z., Libert, C., Foidart, J. M. and Noel, A. 2005. Contribution of host MMP-2 and MMP-9 to promote tumor vascularization and invasion of malignant keratinocytes. FASEB J 19, 234-236.
16 Farabegoli, F., Papi, A. and Orlandi, M. 2011. (-)-Epigallocatechin-3-gallate down-regulates EGFR, MMP-2, MMP-9 and EMMPRIN and inhibits the invasion of MCF-7 tamoxifen-resistant cells. Biosci Rep 31, 99-108.   DOI   ScienceOn
17 Singh, D., Aggarwal, A., Maurya, R. and Naik, S. 2007. Withania somnifera inhibits NF-kappaB and AP-1 transcription factors in human peripheral blood and synovial fluid mononuclear cells. Phytother Res 21, 905-913.   DOI   ScienceOn
18 Thaiparambil, J. T., Bender, L., Ganesh, T., Kline, E., Patel, P., Liu, Y., Tighiouart, M., Vertino, P. M., Harvey, R. D., Garcia, A. and Marcus, A. I. 2011. Withaferin A inhibits breast cancer invasion and metastasis at sub-cytotoxic doses by inducing vimentin disassembly and serine 56 phosphorylation. Int J Cancer 129, 2744-2755.   DOI   ScienceOn
19 Amalinei, C., Caruntu, I. D. and Balan, R. A. 2007. Biology of metalloproteinases. Rom J Morphol Embryol 48, 323-334.
20 Oh, J. H., Lee, T. J., Kim, S. H., Choi, Y. H., Lee, S. H., Lee, J. M., Kim, Y. H., Park, J. W. and Kwon, T. K. 2008. Induction of apoptosis by withaferin A in human leukemia U937 cells through down-regulation of Akt phosphorylation. Apoptosis 13, 1494-1504.   DOI
21 Park, M. J., Park, I. C., Lee, H. C., Woo, S. H., Lee, J. Y., Hong, Y. J., Rhee, C. H., Lee, Y. S., Lee, S. H., Shim, B. S., Kuroki, T. and Hong, S. I. 2003. Protein kinase C-alpha activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells. Int J Oncol 22, 137-143.
22 Ramos-DeSimone, N., Hahn-Dantona, E., Sipley, J., Nagase, H., French, D. L. and Quigley, J. P. 1999. Activation of matrix metalloproteinase-9 (MMP-9) via a converging plasmin/ stromelysin-1 cascade enhances tumor cell invasion. J Biol Chem 274, 13066-13076.   DOI   ScienceOn
23 Shin, M., Yan, C. and Boyd, D. 2002. An inhibitor of c-jun aminoterminal kinase (SP600125) represses c-Jun activation, DNA-binding and PMA-inducible 92-kDa type IV collagenase expression. Biochim Biophys Acta 1589, 311-316.   DOI   ScienceOn
24 Sil, H., Sen, T. and Chatterjee, A. 2011. Fibronectin-integrin (alpha5beta1) modulates migration and invasion of murine melanoma cell line B16F10 by involving MMP-9. Oncol Res 19, 335-348.   DOI