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http://dx.doi.org/10.5352/JLS.2013.23.2.311

Activity-guided Screening of Anti-inflammatory Compounds from the Hexane Extracts of Schisandra chinensis Fruit  

Choi, Hee Jung (Department of Anatomy, School of Medicine, Pusan National University)
Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University)
Baek, Sun-Yong (Department of Anatomy, School of Medicine, Pusan National University)
Kim, Bong-Seon (Department of Anatomy, School of Medicine, Pusan National University)
Ahn, Soon Cheol (Department of Microbiology and Immunology, School of Medicine, Pusan National University)
Rhee, Moon-Soo (Korea Research Institute of Bioscience & Biotechnology)
Yoon, Sik (Department of Anatomy, School of Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.23, no.2, 2013 , pp. 311-318 More about this Journal
Abstract
Schisandra chinensis containing a variety of pharmacologically active lignans has been traditionally used in oriental medicine. In this study, anti-inflammatory compounds were screened from the hexane extracts of S. chinensis by activity-guided fractionation. First, we investigated the regulatory effects on the expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) with 38 fractions from the hexane extracts of S. chinensis in human umbilical vein endothelial cells (HUVECs). As a result, SCKH1 among the 38 fractions from the hexane extract of S. chinensis was selected for further analysis based on its unique regulatory effect on cell adhesion molecules, especially on VCAM-1, in LPS-stimulated HUVECs. The subsequent activity-guided fractionation of SCKH1 resulted in the purification of SCKH1PAIBPB, which was found to suppress the expression of VCAM-1, MCP-1, IL-6 and IL-8 in HUVECs stimulated with LPS, and to inhibit the adhesive capacity between HUVECs and monocytes. Taken together, our data indicate that SCKH1PAIBPB can be proposed as an effective anti-inflammatory compound that may have a potential therapeutic use for the prevention and treatment of various inflammatory diseases as well as ischemic vascular diseases.
Keywords
Activity-guided isolation; anti-inflammatory; cell adhesion molecule; Schisandra chinensis; SCKH1PAIBPB;
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1 Choi, S. R., Kim, C. S., Kim, J. Y., You, D .H., Kim, J. M., Kim, Y. S., Song, E. J., Kim, Y. G., Ahn, Y. S. and Choi, D. G. 2011. Changes of Antioxidant activity and lignan contents in Schisandra chinensis by harvesting times. Korean J Med Crop Sci 19, 414-420.   과학기술학회마을   DOI   ScienceOn
2 Cho, Y. J., Ju, I. S., Kim, B. C., Lee, S. Kim, M. J., Lee, B. G., An, B. J., Kim, J. H. and Kwon, O. J. 2007. Biological activity of omija (Schizandra chinensis baillon) extracts. J. Kor Soc App Bio Chem 50, 198-203.   과학기술학회마을
3 Choi, Y. W., Kim, H. J., Park, S. S., Chung, J. H., Lee, H. W., Oh, S. O., Kim, B. S., Kim, J. B., Chung, H. Y., Yu, B. P., Kim, C. D. and Yoon. S. 2009. Inhibition of endothelial cell adhesion by the new anti-inflammatory agent ${\alpha}$-iso-cubebene. Vascul Pharmacol 51, 215-224.   DOI   ScienceOn
4 Chung, K. H., Lee, S. H., Lee, Y. C. and Kim, J. T. 2001. Antimicrobial activity of Omija (Schizandra chinensis) extracts. J Korean So Food Sci Nutr 30, 127-132.   과학기술학회마을
5 Dinarello, C. A. 2000. Pro-inflammatory cytokines. Chest 118, 503-508.   DOI   ScienceOn
6 Hiroshi, S., Yasuhide, M., Masuko, K. and Tojiro, T. 1996. Effects of some vegetable extracts on the growth of human cell lines measured by the WST-1 assay. Nippon Shokuhin Kagaku Kogaku Kaishi 43, 64-68.   DOI
7 Jeon, T. W., Jo, C. H, Kim, H. and Byun, M. W. 2002. Inhibitory effect on tyrosinase and xanthine oxidase, and nitrite scavenging activities of Schizandrae fructus extract by gamma irradiation. Korean J Food Pre 9, 369-374.   과학기술학회마을
8 Kang, S. Y. and Lee, W. I. 2006. Apolipoprotein E polymorphism in ischemic stroke patients with different pathogenetic origins. Korean J Lab Med 26, 210-216.   과학기술학회마을   DOI   ScienceOn
9 Kim, C. H., Kwon, M .C., Kim, H. S., Ahn, J. H., Chio, G. P., Choi, Y. B., Ko, J. R. and Lee, H. Y. 2007. Enhancement of immune activities of Kadsura Japonica dunal. Through Conventional Fermentation Process. Korean J Med Crop Sci 15, 162-169.   과학기술학회마을
10 Kim, D. G., Kim, M. B., Kim, H., Park, J. H., Im, J. P. and Hong, S. H. 2005. Herb medicinal pharmacognosy. pp. 407, Shinill Books, Seoul, Korea.
11 Kim, J .S. and Choi, S. Y. 2008. Physicochemical properties and antioxidative activities of Omija (Schizandra chinensis Bailon). Korean J Food Nutr 21, 35-42.   과학기술학회마을
12 Kim, O. C. and Jang, H. J. 1994. Volatile components of Schizandra chinensis Baillon. Korean J Agric Biotech 39, 30-36.
13 Lee, C. H., Yi, H. S., Kim, J. E., Heo, S. K., Cha, C. M., Won, C. W. and Park, S. D. 2009. Anti-oxidative and anti-inflammatory effect of fractionated extracts of Smilacis glabrae rhizoma in human umbilical vein endothelial cell. Korean J Herbology 24, 39-50.   과학기술학회마을
14 Lee, S. H., Lee, Y. C. and Yoon, S. K. 2003. Isolation of the antimicrobial compounds from Omija (Schizandra chinensis) extract. Korean J Food Sci Technol 35, 483-487.   과학기술학회마을
15 Mok, C. K. 2005. Quality characteristics of instant tea prepared from spray-dried Omija (Schizandra chinensis Baillon) extract/grape juice mixture. Food Eng Prog 9, 226-230.
16 Lee, Y. J., Shim, J. W,. Lee, Y. J., Parka, Y. H., Lee, H. Y., Kim, S. D., Choi, Y. W. and Bae, Y. S. 2009. Identification of a novel compound that stimulates intracellular calcium increase and CXCL8 production in human neutrophils from Schisandra chinensis. Biochem Biophys Res Comm 379, 928-932.   DOI   ScienceOn
17 Lee, S. K., Kim, S. D., Lee, H. Y., Baek, S. H., Ko, M. J., Son, B. G., Park, S., Choi, Y. W. and Bae, Y. S. 2012. ${\alpha}$-Iso-cubebene, a natural compound isolated from Schisandra chinensis fruit, has therapeutic benefit against polymicrobial sepsis.. Biochem Biophys Res Comm 426, 226-231.   DOI   ScienceOn
18 Liu, C., Zhang, S. and Wu, H. 2009 Non-thermal extraction of effective ingredients from Schisandra chinensis Baill and the antioxidant activity of its extract. Nat Prod Res 23, 1390-1401.   DOI   ScienceOn
19 Oh, S. L., Kim, S. S., Min, B. Y. and Chung, D. H. 1990. Composition of free sugars, free amino acid, non-volatile organic acids and tannins in the extracts of L. chinesis M., A. acutiloba K., S. chinensis B. and A. sessiliflorum S. Korean J Food Sci Technol. 22, 76-81.   과학기술학회마을
20 Piguet, P. E., Grau, G. E., Houser, C. and Vassalli, P. 1991. Tumor necrosis factor is a critical mediators in hapten induced irritant and contact hypersensitivity reaction. J Exp Med 173, 673-679.   DOI   ScienceOn
21 Rong, Y., Geng, Z. and Lau, B. H. 1996. Ginko biloba attenuates oxidative stress in macrophages and endothelial cells. Free Radic Biol Med 20, 121-127.   DOI   ScienceOn
22 Stein, O, Thiery, J. and Stein, Y. 2002. Is there a genetic basis for resistance to atherosclerosis? Atherosclerosis 160, 1-10.   DOI   ScienceOn
23 Ryu, I. H. and Kwon, T. O. 2012. The antioxidative effect and Ingredients of oil extracted from Schizandra chinensis seed. Korean J Med Crop Sci 20, 63-71.   과학기술학회마을   DOI   ScienceOn
24 Sladkovsky, R., Solich, P. and Opletal, L. 2001. Simultaneous determination of quercetin, kaempferol and (E)-cinnamic acid in vegetative organs of Schisandra chinensis Baill. by HPLC. J Pharm Biomed Anal 24, 1049-1054.   DOI   ScienceOn
25 Statistics Korea. "Annual report on the cause of death statistics, 2009". (http://www.kostat.go.kr).
26 Tribolo, S., Lodi, F., Connor, C., Suri, S., Wilson, V. G., Taylor, M. A., Needs, P. W., Kroon, P. A. and Hughes, D. A. 2008. Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells. Atherosclerosis 197, 50-56.   DOI   ScienceOn
27 Yoon, W. Lee, J., Kim, K. N., Kim, J. Y. and Park, S. Y. 2007. In vitro Anti-inflammatory activity of the Artemisia fukudo extracts in murine macrophage RAW 264.7 cells. Korean J Food Sci Technol 39, 464-469.   과학기술학회마을