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http://dx.doi.org/10.5352/JLS.2013.23.2.299

Effect of Glucose on Swarming Motility of Paenibacillus sp. CK214  

Kang, Sung Wan (Department of Microbiology, Pusan National University)
Yoo, Ah Young (Department of Microbiology, Pusan National University)
Kang, Ho Young (Department of Microbiology, Pusan National University)
Publication Information
Journal of Life Science / v.23, no.2, 2013 , pp. 299-305 More about this Journal
Abstract
Paenibacillus is a gram-positive, spore-forming aerobes that was previously classified as a Bacillus species. Paenibacillus sp. CK214 was highly motile on LB agar plates and showed typical colonial morphology of Paenibacillus. However, its motility was defective in the absence of glucose. Electron microscopic observation revealed that the cells of CK214 cultured on LB agar plates were peritrichously flagellated but not flagellated in the presence of glucose. Flagellar filaments were purified by centrifugation after shearing off from the CK214 cells with vigorous pipetting. The purified protein was composed of a single flagellin with an apparent molecular size of 29 kDa. Recognition of the protein by anti-Edwardsiella tarda flagellin protein antibody demonstrates that the protein is a flagellin protein. A decreased level of flagellin protein was detected in CK214 cells grown under glucose-supplemented media.
Keywords
Flagellin; glucose effect; Paenibacillus; swarming;
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1 Aldridge, P. and Hughes, K. T. 2002. Regulation of flagellar assembly. Curr Opin Microbiol 5, 160-5.   DOI   ScienceOn
2 Ash, C., Priest, F. G. and Collins, M. D. 1993. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 64, 253-260.
3 Bertani, G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol 62, 293-300.
4 Deutscher, J. 2008. The mechanisms of carbon catabolite repression in bacteria. Curr Opin Microbiol 11, 87-93.   DOI   ScienceOn
5 Eberl, L., Molin, S. and Givskov, M. 1999. Surface motility of Serratia liquefaciens MG1. J Bacteriol 181, 1703-1712.
6 Fraser, G. M. and Hughes, C. 1999. Swarming motility. Curr Opin Microbiol 2, 630-635.   DOI   ScienceOn
7 Fujihara, M., Maeda, K., Sasamori, E., Matsushita, M. and Harasawa, R. 2009. Effects of chelating reagents on colonial appearance of Paenibacillus alvei isolated from canine oral cavity. J Vet Med Sci 71, 147-153.   DOI   ScienceOn
8 Gorke, B. and Stulke, J. 2008. Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol 6, 613-624.   DOI   ScienceOn
9 Harshey, R. M. 2003. Bacterial motility on a surface: many ways to a common goal. Ann Rev Microbiol 57, 249-273.   DOI   ScienceOn
10 Harshey, R. M. 1994. Bees aren't the only ones: swarming in gram-negative bacteria. Mol Microbiol 13, 389-394.   DOI   ScienceOn
11 Harshey, R. M. and Matsuyama, T. 1994. Dimorphic transition in Escherichia coli and Salmonella typhimurium: surface- induced differentiation into hyperflagellate swarmer cells. Proc Natl Acad Sci USA 91, 8631-8635.   DOI   ScienceOn
12 Ingham, C. J. and Ben Jacob, E. 2008. Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells. BMC Microbiol 8, 36.   DOI   ScienceOn
13 Jones, B. E., Dossonnet, V., Kuster, E., Hillen, W., Deutscher, J. and Klevit, R. E. 1997. Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr. J Biol Chem 272, 26530-26535.   DOI   ScienceOn
14 Kang, S. W., Yoo, A. H., Yu, J. E. and Kang, H. Y. 2012. Characterization and identification of an agar-degrading motile bacteria strain. J Life Sci 22, 259-265.   과학기술학회마을   DOI   ScienceOn
15 Ko, C. H., Tsai, C. H., Lin, P. H., Chang, K. C., Tu, J., Wang, Y. N. and Yang, C. Y. 2010. Characterization and pulp refining activity of a Paenibacillus campinasensis cellulase expressed in Escherichia coli. Bioresour Technol 101, 7882-7888.   DOI   ScienceOn
16 Kohler, T., Curty, L. K., Barja, F., van Delden, C. and Pechere, J. C. 2000. Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili. J Bacteriol 182, 5990-5996.   DOI   ScienceOn
17 Madigan, T., Martinko, J. M., Dunlap, P. V. and Clark, D. P. 2009. Biology of Microorganisms, 10th ed. Pearson Benjamin Cummings, San Francisco.
18 Seidl, K., Muller, S., Francois, P., Kriebitzsch, C., Schrenzel, J., Engelmann, S., Bischoff, M. and Berger-Bachi, B. 2009. Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus. BMC Microbiol 9, 95.   DOI   ScienceOn
19 Rather, P. N. 2005. Swarmer cell differentiation in Proteus mirabilis. Environ Microbiol 7, 1065-1073.   DOI   ScienceOn
20 Sambrook, J. and Russel, D. W. 2001. Molecular Cloning a laboratory manual, 3rd eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
21 Sudo, M., Sakka, M., Kimura, T., Ratanakhanokchai, K. and Sakka, K. 2010. Characterization of Paenibacillus curdlanolyticus intracellular xylanase Xyn10B encoded by the xyn10B gene. Biosci Biotechnol Biochem 74, 58-60.
22 Tcherpakov, M., Ben-Jacob, E. and Gutnick, D. L. 1999. Paenibacillus dendritiformis sp. nov., proposal for a new pattern- forming species and its localization within a phylogenetic cluster. Int J Syst Bacteriol 1, 239-246.
23 Titgemeyer, F. and Hillen, W. 2002. Global control of sugar metabolism: a gram-positive solution. Antonie Van Leeuwenhoek 82, 59-71.   DOI   ScienceOn
24 Towbin, H., Staehelin, T. and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76, 4350-4354.   DOI   ScienceOn
25 Warner, J. B. and Lolkema, J. S. 2003. CcpA-dependent carbon catabolite repression in bacteria. Microbiol Mol Biol Rev 67, 475-490.   DOI   ScienceOn