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http://dx.doi.org/10.5352/JLS.2013.23.2.228

Effects of Medicinal Enzyme Powder on Intestinal Mobility, Lipid Level, and Blood Parameters of Loperamide-Induced Constipation in Rats  

Park, Chan Sung (Faculty of Herbal Food Cooking & Nutrition, Daegu Haany University)
Park, Kyung Soo (Faculty of Herbal Food Cooking & Nutrition, Daegu Haany University)
Kim, Mi Lim (Faculty of Herbal Food Cooking & Nutrition, Daegu Haany University)
Kong, Hyun Joo (Faculty of Herbal Food Cooking & Nutrition, Daegu Haany University)
Yang, Kyung Mi (Faculty of Herbal Food Cooking & Nutrition, Daegu Haany University)
Publication Information
Journal of Life Science / v.23, no.2, 2013 , pp. 228-236 More about this Journal
Abstract
This study was aimed at investigating whether dietary therapy using medicinal enzyme powder is effective in reducing constipation caused by loperamide in rats. Nine-week-old male Sprague Dawley were subdivided into 4 groups: normal diet group (C), loperamide treatment and normal diet (CL), medicinal enzyme powder diet (E), and loperamide treatment and medicinal enzyme powder diet (EL). Constipation was induced by subcutaneous injection of loperamide (1.5 mg/kg) 3 days prior to sacrifice. The treatment with loperamide led to an increase in weight gain, a decrease in the number and wet weight of fecal pellets, and a decrease in intestinal motility. The administration of the medicinal enzyme powder significantly reduced weight gain but increased intestinal mobility compared with the loperamide-treated group. The treatment with loperamide in the normal diet group reduced the activities of both suggesting that constipation may be involved in the low level of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT). Additionally, the loperamide treatment in the medicinal enzyme powder diet group increased the level of GOT, but reduced the level of GPT. Loperamide treatment also reduced cholesterol and increased the atherogenic index (AI) and cardiac risk factors (CRFs). Interestingly, the treatment with the medicinal enzyme powder effectively attenuated both the increase in AI and the reduction in high density lipopretein (HDL)-cholesterol, caused by the treatment with loperamide. Although there were no significant differences in the blood protein level, including hemoglobin and hematocrit, between the normal diet group and the loperamide-treated group, the administration of the medicinal enzyme powder to the loperamide-treated group effectively increased the levels of both hemoglobin and hematocrit. Collectively, the results demonstrate that the medicinal enzyme powder can help to combat the negative events caused by constipation.
Keywords
Medicinal enzyme powder; constipation; intestinal motility; lipid level; blood parameter;
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Times Cited By KSCI : 14  (Citation Analysis)
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1 Baek, Y. H. 2005. A study of dietetic on the constipation. Korean J Herbology 20, 181-191.   과학기술학회마을
2 Bang, M. A., Cho, Y. J. and Kim, H. A. 2002. Effect of Indongcho (L. Japonica Thunb) on glucose and lipid metabolism and antioxidative enzyme system in streptozotocin- induced diabetic rats. Korean J Dietary Culture 17, 377-386   과학기술학회마을
3 Burkitt, D. P., Waker, A. R. P. and Painter, N. S. 1974. Dietary fiber and disease. J Am Med Assoc 229, 1068-1074.   DOI   ScienceOn
4 Cho, Y. S., Kim, S. I. and Han, Y. S. 2008. Effects of Slander Glasswort (Salicornia herbacea L.) extract on improvements in bowel function and constipation relief. J Food Sci 40, 326-331.   과학기술학회마을
5 Corazziari, E. 1999. Need of the drug for the treatment of chronic constipation. Ital J Gasteroenterol 31, 232-133.
6 Fadel, J. G., Newman, R. K., Newman, C. W. and Barres, A. E. 1987. Hypo- cholesterol effects of ${\beta}$-glucans in different barley diets fed to brolier chicks. Nutr Rep Int 35, 1049-1053.
7 Friedewald, W. T., Levy, R. I. and Fedreicson, D. S. 1972. Estimation of concentration of low density lipoprotein cholesterol in plasma without use of the preperative ultracentrifuge. Clin Chem 18, 449-502.
8 Fuller, R. 1989. Probiotics in man and animal. J Appl Bacteriol 66, 365-378.   DOI
9 Haglund, O., Loustarinen, R., Wallin, R., Wibell, I. and Sadeen, T. 1991. The effect of fish oil on triglyceride, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur J Nutr 121, 165-172.
10 Ji, H. J. 1999. Health foods from herbs. Department of food science and nutrition. Ph. D. dissertation. Seoul National University. Seoul, Korea.
11 Jung, J. M., Kim, S. Y. and Jang, S. W. 2007. The power of life ․ The mystery of the enzyme. pp. 58-60, MD Journal. Seoul, Korea.
12 Kawanuchi, M., Tashiro, Y., Adachi, T. and Tamura, Z. 1993. Changes in intestinal condition, fecal microflora and composition of rectal gas after administration of fructooligosaccharide and lactulose at different doses. Bifidibacteria Microflora 12, 57-67.   DOI
13 Kim, D. J., Oh, S. K., Yoon, M. R., Hong, H. C., Lee, J. S. and Kim, Y. K. 2010. Antioxidant compounds and antioxidant activities of the 70% ethanol extracts from brown and milled rice by culivar. J Korean Soc Food Sci Nutr 39, 467-473.   DOI   ScienceOn
14 Kim, H. C. 2010. Modern people should eat enzyme like rice. pp. 86-90, Sogum Namu, Seoul Korea.
15 Kim, H. C., 2010. Modern people should eat enzyme like rice. pp. 49-50, Sogum Namu, Seoul Korea.
16 Kim, J. Y., Seo, Y. J., Noh, S. K. and Cha, Y. J. 2010. A concentrated onion extract lowers serum lipid levels in rats fed a high-fat diet. Korean J Food Preserv 17, 398-404.   과학기술학회마을
17 Kim, J. D. 2012. Literature on the quality and effect of Job's Tears (Coix lachryma -jobi L. Var. mayuen S). J Korean Agricultural History Association 11, 89-121.
18 Kim, K. H., Chang, J. S. and Oh, Y. K. 2011. Nutrient digestibilities and fecal characteristics of diets including brown rice for Miniature Schnauzer. J Animal Science Tech 53, 429-434.   과학기술학회마을   DOI   ScienceOn
19 Kim, J. E., Kim, A. R., Kim, M. J. and Park, S. N. 2011. Antibacterial, antioxidative and antiaging effects of Allium cepa L. peel extracts. Appl Chem Eng 22, 178-184.
20 Kim, J. Y., Kim, O. Y., Yoo, H. J., Kim, T. I., Kim, W. H., Yoon, Y. D. and Lee, J. H. 2006. Effects of fiber supplements on functional constipation. Korean J Nutr 39, 35-43   과학기술학회마을
21 Kim, O. K. 2008. Antidiabetic effect of Ha-Su-O (polygoni radix). J Korean Oil Chemists Soc 25, 347-354.   과학기술학회마을
22 Lee, H. J., Byun, S. M. and Kim, H. S. 1988. Studies on the dietary fiber of brown rice and milled rice. Korean J Food Sci Technol 20, 576-584.   과학기술학회마을
23 Lee, J. M., Lee, S. H., Lee, J. H. and Lee, E. 2006. Effects of Coix lachryma-jobi var. mayuen stapf. pharmacopuncture on plasma lipid composition and glucose in rat fed high fat diet. Korean J Meridian Acupoint 23, 59-86.   과학기술학회마을
24 Lee, J. J., Lee, Y. M., Jung, S. K., Kim, K. Y. and Lee, M. Y. 2008. Effects of dietary Mulberry Leaf in loperamide-induced constipation in rats. Korean Soc Food Preservation 15, 280-287.   과학기술학회마을
25 Martelli, H., Devroede, G., Arhan, P., Duguay, C., Dornic, C. and Faverdin, C. 1978. Some parameters of large bowel motility in normal man. J Gasteroenterology 75, 612-618.
26 Mo, J. H. and Kim, K. R. 2011. The proximate and antioxidative effect of dried and fermented Salicornia herbacea. Korean J Soc Trichologists 7, 425-431
27 Park, M. K., Lee, J. M., Park, C. H. and In, M. J. 2002. Quality characteristics of Sulgidduk containing chlorella powder. J Korean Soc Food Sci Nutr 31, 225-229.   과학기술학회마을   DOI   ScienceOn
28 Park, H. J., Lee, C. H., Chung, J. P., Lee, K. S. and Lee, S. I. and Park, I. S. 1994. Clinical study of chronic idopathic constipation. Korean J Med 46, 670-680.
29 Park, J. Y., Jang, J. Y., Lee, M. K., Park, E. M., Kim, M. J., Son, D. H., Chung, H. C. and Cho, S. Y. 2002. Effect of Dandelion (Taraxacum Officinale) extracts on the intestinal microorganism of streptozotocin-induced diabetic rats. J Korean Food Sci Nutr 31, 1112-1118.   DOI   ScienceOn
30 Park, M. I. 2011. Treatment of constipation. Korean J Med 80, 510-523.
31 Park, M. K., Jin, Y. G., Kim, D. G., Jin, J. Y. and Lee, Y. J. 2007. Effects of Lentinus edodes extract on the loperamide- induced constipation in rats. Korean J Food Sci Nutr 39, 88-93.   과학기술학회마을
32 Sandler, R. S. and Drossman, D. A. 1987. Bowel habits in young adults not seeking health care. Dig Dis Sci 32, 841-845.   DOI   ScienceOn
33 Schiller, L. R., Sanat, S. B., Ana. C. A., Moravski, S. G. and Fordtran, J. S. 1984. Mechanism of the antidiarrheal action of loperamide. J Gastroenterology 86, 1475-1480.
34 Seo, B. I., Park, J. H., Choi, H. S., Kim, S. M., Gu, D. M., Kim, M. R. and Park, J. H. 2008. Effects of water extracts from Lagocephalus wheeleri with several herbs on hyperlipidemia and liver damage. Korean J Herbology 23, 9-15.
35 Shimotoyodome, A., Meguro, S., Hase, T., Tokimitsu I. and Sakata, T. 2000. Decreased colonic mucus in rats with loperamide- induced constipation. Comp Biochem Physiol A Mol Integr Physiol 126, 203-212.   DOI   ScienceOn
36 Szajewska, H., Setty. M., Mrukowicz, J. and Guandalini, S. 2006. Probiotics in gastrointestinal disease in children: hard and non-so-hard evidence of efficacy. J Pediatr Gast Nutr 42, 454-475.   DOI   ScienceOn
37 Sin, H. J. 2008. Effect of dietary resistant starch on the large bowel function in rats with loperamide-induced constipation. Department of food science and nutrition, MS. dissertation. Kyungpook National University. Daegu, Korea.
38 Slavin, J., Jacobs, D. and Marquart, L. 1997. Whole-grain consumption and chronic disease ; protective mechanism. Nutr Cancer 27, 14-21.   DOI   ScienceOn
39 Stone, B. A. 1986. Cell walls and their components in cereal grain technology. Am Assoc Cereal Chem 8, 116-122.
40 Tanaka, K., Aso, B. and Sugano, M. 1984. Biliary steroid excretion in rats fed soybean protein and casein or their amino acid mixtures. J Nutr 112, 26-32
41 Warren, J. R. 1983. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. J Lancet 1, 1273-1275.
42 Woo, J. H., Choi, I. Y. and Choue, R. W. 2007. Hematology effects of water extracts of Cham-Dang-Gui on dietary induced iron deficient anemia rat. Korean J Nutr 40, 428-434.
43 Yang, J. L., Lee, S. H. and Song, Y. S. 2003. Improving effect of powders of cooked soybean and chongkukjang on blood pressure and lipid metabolism in spontanecously hypertensive rats. J Korean Soc Food Sci Nutr 32, 899-905.   DOI   ScienceOn