Browse > Article
http://dx.doi.org/10.5352/JLS.2013.23.1.31

Effect of Hydropsyche kozhantschikovi Extracts on Oxidative Stress  

Park, Young Mi (Department of Biological Science, Andong National University)
Lim, Jae Hwan (Department of Biological Science, Andong National University)
Lee, Jong Eun (Department of Biological Science, Andong National University)
Seo, Eul Won (Department of Biological Science, Andong National University)
Publication Information
Journal of Life Science / v.23, no.1, 2013 , pp. 31-37 More about this Journal
Abstract
The present study aimed to investigate effects of ethanol extracts from Hydropsyche kozhantschikovi on cell and DNA damage caused by oxidative stress. In a radical scavenging assay, compared with ascorbic acid used as a control, the level of DPPH (1,1-diphenyl-2-picrylhydrazyl) and that of hydroxyl radicals in H. kozhantschikovi extracts were 60.0% and 43.7%, respectively. The ferrous iron chelating level was 37.5% compared to the chelating value of EDTA (ethylenediaminetetraacetic acid) as a positive control at the same concentration. To verify inhibitory effects of oxidative cell damage induced by reactive oxygen species (ROS), the relative level of lipid peroxidation and the expression level of the p21 protein were compared in extracts-treated and untreated groups. Lipid peroxidation was completely inhibited in the extracts-treated group compared with the radical-only treated group. The level of p21 protein expression was restored to 92.2% of p21 protein expression in the control sample. In addition, DNA cleavage inhibition in the H. kozhantschikovi extracts was 74.1% compared with that of the control group, suggesting that H. kozhantschikovi extracts repress DNA cleavage induced by ROS. Moreover, the phosphorylation ratio of the H2AX protein was 16.7% in the radical-treated group, indicating that the ethanol extracts inhibited 83.3% of DNA damage. Our findings suggest that ethanol extracts from H. kozhantschikovi are effective not only in repressing the oxidation of free radicals and highly toxic hydroxyl radicals, but also in decreasing cell and DNA damage caused by oxidative stress.
Keywords
Antioxidant activity; Hydropsyche kozhantschikovi; oxidative stress; radical;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Hong, S. C., Jeong, J. B. and Jeong, J. H. 2008. Buddleja officinalis prevents the normal cells from oxidative damage via antioxidant activity. Korean J Plant Res 21, 449-456.   과학기술학회마을
2 Heo, J. C., Park, J. Y., Hwang, J. S., Park, H. C., Kang, S. W., Hwang, S. J., Yun, C. Y., Kwon, T. K. and Lee, S. H. 2006. Comparison of in vitro antioxidant activity and Cyclooxygenase-2 promoter inhibitory activity in Harmonia axyridis Pallas and Coccinella septempunctata Linne. Korean J Food Preserv 13, 513-518.
3 Hsu, B., Coupar, I. M. and Ng, K. 2006. Antioxidant activity of hot water extract from the fruit of the doum palm, Hyphaene thebaica. Food Chem 98, 317-328.   DOI   ScienceOn
4 Jeong, J. B., Hong, S. C. and Jeong, H. J. 2009a. 3, 4-Dihydroxybenzaldehyde purified from the barley seeds (Hordeum vulgare) inhibits oxidative DNA damage and apoptosis via its antioxidant activity. Phytomedicine 16, 85-94.   DOI   ScienceOn
5 Jeong, I. Y., Lee, J. S., Oh, H., Jeong, U. H., Park, H. R. and Jo, S. K. 2003. Inhibitory effect of hot-water extracts of Paeonia japonica on oxidative stress and identification of its active components. J Korean Soc Food Sci Nutr 32, 739-744.   DOI   ScienceOn
6 Jeong, J. B., Park, J. H., Lee, H. K., Ju, S. Y., Hong, S. C., Lee, J. R., Chung, G. Y., Lim, J. H. and Jeong, H. J. 2009. Protective effect of the extracts from Cnidium officinale against oxidative damage induced by hydrogen peroxide via antioxidant effect. Food Chem Toxicol 47, 525-529.   DOI   ScienceOn
7 Kang, K. A., Zhang, R., Piao, M. J., Ko, D. O., Wang, Z. H., Kim, B. J., Park, J. W., Kim, H. S., Kim, D. H. and Hyun, J. W. 2008. Protective effect of irisolidone, a metabolite of kakkalide, against hydrogen peroxide induced cell damage via antioxidant effect. Bioorg Med Chem 16, 1133-1141.   DOI   ScienceOn
8 Kim, J. W., Moon, B. S., Park, Y. M., Yoo, N. H., Ryoo, I. J., Nguyen, T. C., Yoo, I. D. and Kim, J. P. 2005. Structures and antioxidant activity of diketopiperazines isolated from the mushroom Sarcodon aspratus. J Korean Soc Appl Biol Chem 48, 93-97.   과학기술학회마을
9 Kim, K. M., Park, C., Choi, Y. H. and Lee, W. H. 2008. Induction of apoptosis by water extract of Cordyceps militaris (WECM) in human hepatocellular carcinoma HepG2 Cells. J Life Sci 18, 804-813.   DOI   ScienceOn
10 Lightfoot, T., Skibola, C. F., Smith, A. G., Forrest, M. S., Adamson, P. J., Morgan, G. J., Bracci, P. M., Roman, E., Smith, M. T. and Holly, E. Z. 2006. Polymorphisms in the oxidative stress genes, superoxide dismutase, glutathione peroxidase and catalase and risk of non-Hodgkin’s lymphoma. Br J Haematol 91, 1222-1227.
11 Looi, M. L., Mohd Dali, A. Z., Md Ali, S. A., Wan Ngah, W. Z. and Mohd Yusof, Y. A. 2008. Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix. Eur J Cancer Prev 17, 555-560.   DOI   ScienceOn
12 Martinez, G. R., Loureiro, A. P. M., Marques, S. A., Miyamoto, S., Yamaguchi, L. F., Onuki, J., Almeida, E. A., Garcia, C. C. M., Barbosa, L. F., Medeiros, M. H. G. and Di Mascio, P. 2003. Oxidative and alkylating damage in DNA. Mutat Res 544, 115-127.   DOI   ScienceOn
13 McKee, T. and McKee, J. R. 2002. Biochemistry: the molecular basis of life. McGraw-Hill, New York.
14 Park, S. Y. and Chin, K. B. 2007. Evaluation of antioxidant activity in pork patties containing bokbunja (Rubus coreanus) extract. Korean J Food Sci Ani Resour 27, 432-439.   과학기술학회마을   DOI   ScienceOn
15 Park, J. Y., Heo, J. C., An, S. M., Yun, E. Y., Han, S. M., Hwang, J. S., Kang, S. W., Yun, C. Y. and Lee, S. H. 2005. High throughput-compatible screening of anti-oxidative substances by insect extract library. Korean J Food Preserv 12, 482-488.
16 Park, M. H., Kang, S. M., Jung, H. Y. and Hong, S. G. 2003. Protecting effects of vitamine E against immobilization stress-induced oxidative damage in rat brain. Korean J Nutr 36, 570-576.
17 Park, J. Y., Heo, J. C., Woo, S. U., Yun, C. Y., Kang, S. W., Hwang, J. S. and Lee, S. H. 2006. Anti-inflammatory and cellular protective effects on hydrogen peroxide-induced cytotoxicity of grasshopper extracts. Korean J Food Preserv 13, 796-802.   과학기술학회마을
18 Park, Y. M., Jeong, J. B., Seo, J. H., Lim, J. H., Jeong, H. J. and Seo, E. W. 2011. Inhibitory effect of red bean (Phaseolus angularis) hot water extracts on oxidative DNA and cell damage. Korean J Plant Res 24, 130-138.   과학기술학회마을   DOI   ScienceOn
19 Park, Y. M., Lim, J. H., Jeong, H. J. and Seo, E. W. 2011. Effect of Schizandra chinensis extracts on oxidative damage. J Exp Biomed Sci 17, 69-77.   과학기술학회마을
20 Park, Y. M., Kim, J. I., Lee, C. H., Lim, J. H. and Seo, E. W. 2011. Effect of Dendranthema indicum extracts on cell and DNA damage induced by oxidative stress. J Life Sci 21, 1698-1704.   과학기술학회마을   DOI   ScienceOn
21 Punchard, N. A. and Kelly, F. J. 1996. A practical approach, pp. 1-8, In Punchard, N. A. and F. J. Kelly (eds.), Free radicals. Oxford University Press, New York.
22 Smirnoff, N. and Cumbes, Q. J. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28, 1057-1060.   DOI   ScienceOn
23 Thannickal, V. J. and Fanburg, B. L. 2000. Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 279, 1005-1029.
24 Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M. and Telser, J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39, 44-84.   DOI   ScienceOn
25 Yoon, W. J., Lee, J. A., Kim, J. Y., Kim, S. B. and Park, S. Y. 2007. Antioxidant activity and physiological function of the Anomala albopilosa extracts. J Korean Soc Food Sci Nutr 36, 670-677.   과학기술학회마을   DOI   ScienceOn
26 You, H. J., Oh, D. H, Choi, C. Y., Lee, D. G., Hahm, K. S., Moon, A. R. and Jeong, H. G. 2002. Protective effect of metallothionein-III on DNA damage in response to reactive oxygen species. Biochim Biophys Acta 1573, 33-38.   DOI   ScienceOn