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http://dx.doi.org/10.5352/JLS.2013.23.12.1557

Detection of Carbonic Anhydrase in the Gills of Rainbow Trout (Oncorhynchus mykiss)  

Kim, Soo Cheol (Department of Biotechnology, College of Engineering, Chonnam National University)
Choi, Kap Seong (Department of Food Science and Technology, Sunchon National University)
Kim, Jung Woo (Department of Anatomy, College of Medicine, Seonam University)
Choi, Myeong Rak (Department of Biotechnology, College of Engineering, Chonnam National University)
Han, Kyeong Ho (Department of Aquatic Biology, College of Fisheries and Ocean Sciences, Chonnam National University)
Lee, Won Kyo (Department of Aquatic Biology, College of Fisheries and Ocean Sciences, Chonnam National University)
Kho, Kang Hee (Department of Aquatic Biology, College of Fisheries and Ocean Sciences, Chonnam National University)
Publication Information
Journal of Life Science / v.23, no.12, 2013 , pp. 1557-1561 More about this Journal
Abstract
Carbonic anhydrase isozymes are a widespread, zinc-containing metalloenzyme family. The enzyme catalyzes the reversible inter-conversion of $CO_2$ and $HCO_3$. This reaction is the main role played by CA enzymes in physiological conditions. This enzyme has been found in virtually all organisms, and at least 16 isozymes have been isolated in mammals. Unlike mammals, there is little information available regarding CA isozymes in the tissues of non-mammalian groups, such as fish. Carbonic anhydrase is very important in the osmotic and acid-base regulation in fish. It is well-known that the gills of fish play the most important role in acid-base relevant ion transfer, the transfer of $H^+$ and/or $HCO_3^-$, for the maintenance of systemic pH. Rainbow trout, Oncorhynchus mykiss, is the most important freshwater fish species in the aquaculture industry of Korea, with annual production increasing each year. In addition, environmental toxicology research has shown that rainbow trout is known to be the species that is most susceptible to environmental toxins. Consequently, carbonic anhydrase was detected in rainbow trout, Oncorhynchus mykiss. The isolated protein showed the specific band with a molecular weight of 30 kDa and pI of 7.0, and it was identified as being carbonic anhydrase. The immunohistochemical result demonstrated that the carbonic anhydrase was located in the epithelial cells of the gills.
Keywords
Carbonic anhydrase; gill; immunohistochemistry; rainbow trout;
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