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http://dx.doi.org/10.5352/JLS.2013.23.12.1509

Effects of High-fat Diet on Type-I Muscle Loss in Rats  

Baek, Kyung-Wan (Division of Sports Science, Pusan National University)
Cha, Hee-Jae (Genetic Laboratory, Kosin University College of Medicine)
Park, Jung-Jun (Division of Sports Science, Pusan National University)
Publication Information
Journal of Life Science / v.23, no.12, 2013 , pp. 1509-1515 More about this Journal
Abstract
The term lipotoxicity has been used to describe how excess lipid accumulation leads to cellular dysfunction and death in non-adipose tissues, including skeletal muscle. While lipotoxicity has been found in cultured skeletal muscle cells with high-fat feeding, the consequences of lipotoxicity in vivo are still unknown, particularly in Type-I muscle, which is metabolically affected by lipotoxicity. The aim of this study was to investigate the effects of a high-fat diet on changes in the morphology and apoptotic protein expression of Type-I muscle loss in rats. The rats were fed either a high-fat diet or a normal diet for six weeks, and then lipid accumulation, inflammation response, and nucleus infiltration were measured, and PARP protein expression was cleaved by Oil Red O staining, H & E staining, and Western blot, respectively. Lipid accumulation, inflammation response, nucleus infiltration, and cleaved PARP protein expression were significantly (p<0.05) higher in the high-fat diet group than they were in the normal diet group. The weight of Type-I muscle tended to be lower in the high-fat diet group compared to the normal diet group, but the difference was not statistically significant. These results indicate that a high-fat diet triggers cell death in Type-I muscle via lipotoxicity, which suggests that a high-fat diet may be associated with sarcopenia.
Keywords
High-fat diet; cleaved PARP; muscle loss; sarcopenia; Type-I muscle;
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