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http://dx.doi.org/10.5352/JLS.2013.23.12.1420

Synergistic Effect of Acetylalginate Esterase and Alginate Lyase on the Degradation of Acetylalginate from Pseudomonas aeruginosa ATCC 39324  

Kim, Hee Sook (Department of Food Science and Biotechnology, Kyungsung University)
Publication Information
Journal of Life Science / v.23, no.12, 2013 , pp. 1420-1427 More about this Journal
Abstract
A novel acetylalginate esterase (AcAlgE) gene was previously cloned and characterized from Sphingomonas sp. MJ-3. In this study, the synergistic effects of MJ-3 AcAlgE, and KS-408 alginate lyase on the degradation of acetylalginate from Pseudomonas aeruginosa were investigated by using high-field 1H-NMR and an FPLC-equipped peptide column. The alginate lyase coupled assay of AcAlgE showed that degradation of high molecular weight acetylalginate was more difficult than degradation of acid hydrolyzed acetylalginate. The degradation of acetylalginate by alginate lyase was easier after AcAlgE was used to remove the acetyl group from acetylalginate. This result showed that the recombinant AcAlgE enhanced the degradation of acetylalginate by alginate lyase.
Keywords
Acetylalginate esterase (AcAlgE); alginate lyase; acetylalginate; alginate lyase coupled assay;
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1 Park, Y. J., Chu, Y. J., Shin, Y. H., Lee, E. Y. and Kim, H. S. 2013. Molecular cloning and characterization of a novel acetylalginate esterase gene in alg operon from Sphingomonas sp. MJ-3. Appl Microbiol Biotechnol July, [Epub ahead of print].
2 Biely, P., Mastihubová, P., Tenkanen, M., Eyzaguirre, J., Li, X. L. and Vršmanská, M. 2011. Action of xylan deacetylating enzymes on monoacetyl derivatives of 4-nitrophenyl glycosides of $\beta$-D-xylopyranose and $\alpha$-L-arabinofuranose. J Biotechnol 151, 137-142.   DOI   ScienceOn
3 Grasdalen, H. 1983. High-field, $^{1}n$.m.r. spectroscopy of alginate: sequential structure and linkage conformations. Carbohydr Res 118, 255-260.   DOI   ScienceOn
4 Hatch, R. A. and Schiller, N. L. 1998. Alginate lyase promotes diffusion of aminoglycosides through the extracellular polysaccharide of mucoid Pseudomonas aeruginosa. Antimicrob Agents Chemother 42, 974-977.
5 Haug, A., Larsen, B. and Smidsrot, O. 1966. A study of the constitution of alginic acid by partial acid hydrolysis. Acta Chem Scand 20, 183-190.   DOI
6 Hay, I. D., Gatland, K., Campisano, A., Jordens, J. Z. and Rehm, B. H. A. 2009. Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain. Appl Environ Microbiol 75, 6022-6025.   DOI   ScienceOn
7 Kam, N., Park, Y. J., Lee, E. Y. and Kim, H. S. 2011. Molecular identification of a polyM-specific alginate lyase from Pseudomonas sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate. Can J Microbiol 57, 1032-1041.   DOI   ScienceOn
8 May, T. B. and Chakrabarty, A. M. 1994. Isolation and assay of Pseudomonas aeruginosa alginate. Methods Enzymol 235, 295-304.   DOI
9 McComb, E. A. and McCready, R. M. 1957. Determination of acetyl in pectin and in acetylated carbohydrate polymers. Anal Chem 29, 819-821.   DOI
10 Nivens, D. E., Ohman, D. E., Williams, J. and Franklin, M. J. 2001. Role of alginate and its O acetylation in formation of Pseudomonas aeruginosa microcolonies and biofilms. J Bacteriol 183, 1047-1057.   DOI   ScienceOn
11 Pedersen, S. S., Hoiby, N., Espersen, F. and Koch, C. 1992. Role of alginate in infection with mucoid Pseudomonas aeruginosa in cystic-fibrosis. Thorax 47, 6-13.   DOI   ScienceOn
12 Shevechik, V. E., Robert-Baudouy, J. H. and Huguvieux- Cotte-Pattat, N. 2003. Pectate lyase Pel1 of Erwinia chrysanthemi 3937 belongs to a new family. J Bacteriol 185, 3091-3100.   DOI
13 Skjåk-Braek, G., Grasdalen, H. and Larsen, B. 1986. Monomer sequence and acetylation pattern in some bacterial alginates. Carbohydr Res 154, 239-250.   DOI   ScienceOn
14 Sutherland, I. W. and Keen, G. A. 1981. Alginase from Beneckea pelagia and Psudomonas spp. J Appl Biochem 3, 48-57.
15 Uwe, R. and Rehm, B. H. A. 2006. Bacterial alginate : from biosynthesis to applications. Biotechnol Lett 28, 1701-1712.   DOI
16 Veeranagouda, Y., Basavaraja, C. Bae, H.-S., Liu, K.-H. and Lee, K. 2011. Augmented production of poly-${\beta}$-D-mannuronate and its acetylated forms by Pseudomonas. Proc Biochem 46, 328-334.   DOI   ScienceOn
17 Weissbach, A. and Hurwitz, J. 1959. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. J Biol Chem 234, 705-709.
18 Wong, T. Y., Preston, L. A. and Schiller, N. L. 2000. Alginate lyase: Review of major sources and enzume characteristics, structure-function analysis, biological roles, and application. Annu Rev Microbiol 54, 289-340.   DOI   ScienceOn
19 Yu, J., Penatroza-Vazquea, P., Chakrabarty, A. M. and Bender, C. L. 1999. Involvement of the exopolysaccharide alginate in the virulence and epiphytic fitness of Pseudomonas syringae pv. Syringae. Mol Microbiol 33, 712-720.   DOI   ScienceOn
20 Zhang, Z., Yu, G., Guan, H., Zhao, X., Du, Y. and Jiang, X. 2004. Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrio sp. 510. Carbohydr Res 258, 1475-1481.
21 Alipour, M., Suntres, Z. E. and Omri, A. 2009. Importance of DNase and alginate lyase for enhancing free and liposome encapsulated aminoglycoside activity against Pseudomonas aeruginosa. J Antimicrob Chemother 64, 317-325.   DOI   ScienceOn
22 ASTM International. 2010. ASTM F2259-10. Standard test method for determining the chemical composition and sequence in alginate by proton nuclear magnetic resonance (1H NMR) spectroscopy. ASTM International, West Conshohocken, Penn., USA. from http://www.astm.org/ Standards.