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http://dx.doi.org/10.5352/JLS.2013.23.11.1351

Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells  

Lee, Sun Min (Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Kim, Young Sook (Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Jang, Wook Jin (Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Rakib, Abdur Md. (Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi)
Oh, Tae Woo (Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Kim, Boh Hyun (Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Kim, So Young (School of Food Science, International University of Korea)
Kim, Jeong Ok (HK Biotech Co. Ltd.)
Ha, Yeong Lae (Division of Applied Life Science (BK21 Plus), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.23, no.11, 2013 , pp. 1351-1359 More about this Journal
Abstract
The anti-proliferative activity of ${\beta}$-ionone was investigated on human non-small lung cancer A-549 cells (designated A-549 cells). A-549 cells were treated with various concentrations of ${\beta}$-ionone (1, 5, 10, and 15 ${\mu}M$) for two, four, and six days. Biochemical markers related to the growth inhibition of A-549 cells by ${\beta}$-ionone were measured at the second day of incubation. ${\beta}$-Ionone inhibited the growth of A-549 cells by dose-and time-dependent manners, resulting in an $IC_{50}$ of 5.0 ${\mu}g/ml$ at the second day of incubation. ${\beta}$-Ionone induced apoptosis by a dose-dependent manner. ${\beta}$-Ionone increased levels of p53, p21, and Bax proteins, but suppressed expression of the Bcl-2 protein. Similarly, ${\beta}$-ionone enhanced cytochrome c release from the mitochondria to the cytosol, and induced activation of caspase-9 and -3. Additionally, ${\beta}$-ion-one reduced $cPLA_2$ and COX-2 protein levels. These results suggest that the ${\beta}$-ionone inhibits the proliferation of A-549 cells through reciprocal regulation of Bax and Bcl-2 gene expression and suppression of $cPLA_2$ and COX-2 protein expressions.
Keywords
${\beta}$-Ionone; human non small lung cancer A-549 cells; apoptosis; $cPLA_2$; Cox-2;
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