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http://dx.doi.org/10.5352/JLS.2012.22.8.1099

Lack of Cytotoxicity of the Colorant in Conjugated Linoleic Acid against Human Cancer and Normal Cells  

Ji, Yu-Chul (Division of Applied Life Sciences (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Ahn, Chae-Rin (Division of Applied Life Sciences (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Seo, Yang-Gon (Department of Chemical & Bioengineering, College of Engineering, Gyeongsang National University)
Suh, Jeong-Se (Department of Mechanical Engineering, Engineering Research Institute, College of Engineering, Gyeongsang National University)
Kim, Jeong-Ok (HK Biotech Co., Ltd.)
Ha, Yeong-Lae (Division of Applied Life Sciences (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.22, no.8, 2012 , pp. 1099-1106 More about this Journal
Abstract
The cytotoxicity of the colorant in conjugated linoleic acid (CLA) was investigated in human cancer cell lines and a normal human cell line. Commercially-available CLA with a brown color (designate crude CLA; c-CLA) was distilled in a vacuum (10 mmHg-$220^{\circ}C$, 10 mmHg-$235^{\circ}C$, 10 mmHg-$240^{\circ}C$, and 20 mmHg-$260^{\circ}C$) for 30 min to obtain pure CLA (distilled CLA; d-CLA) and dark brown-colored CLA (residual CLA; r-CLA) samples. No color intensity was shown in the d-CLA sample obtained under 10 mmHg-$220^{\circ}C$ conditions of distillation when the L (brightness), a (red/blue), and b (yellow/green) parameters were analyzed, whereas the r-CLA sample showed a dark brown color. The composition of CLA isomers in both the d- and r-CLA samples, as compared to that of the c-CLA sample, was not significantly different when analyzed by gas chromatography. When the cytotoxicity of the r-CLA and d-CLA samples obtained under 10 mmHg-$220^{\circ}C$ conditions were compared against human breast cancer cells (MCF-7), human lung cancer cells (A-549), human colon cancer cells (HT-29), human prostate cancer cells (PC-3), and human neuroblastoma cells (SK-N-SH), no significant cytotoxicity was seen in the cell lines. These results suggest that the color or colorant in the CLA samples did not have any effects on the proliferation of human cancer and normal cells and imply that the colorant in commercially available CLA samples is safe for human consumption.
Keywords
Conjugated linoleic acid; cytotoxicity; human cancer cells; human neuroblastoma cells; distillation;
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