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http://dx.doi.org/10.5352/JLS.2012.22.7.987

Evaluation of the Resistance of Mungbean Lines to Sprout Rot Caused by Pseudomonas species  

Velusamy, Vijayanand (School of Biotechnology, Yeungnam University)
Park, Eui-Ho (School of Biotechnology, Yeungnam University)
Publication Information
Journal of Life Science / v.22, no.7, 2012 , pp. 987-990 More about this Journal
Abstract
Mungbean sprout rot is one of the most serious problems of the commercial mungbean sprout industry. In this study, 70 strains of mungbean sprout rot pathogens were isolated from rotten sprouts at different time intervals. The pathogenicity of the isolated pathogens was tested. The highly pathogenic strain (YV-St-033) was identified as Pseudomonas sp. by 16S rRNA gene sequencing. In phylogenetic analysis, the YV-St-033 strain was grouped with P. mosselii, P. putita, P. fluorescens, P. entomophila, and P. lecoglossicida. The results of the 16S rRNA gene sequence analysis revealed that the YV-St-033 strain shared the highest sequence identity (more than 99%) with the P. mosselii R10 strain. The mungbean lines of Yeungnam University germplasm were screened against the YV-St-033 strain. Based on the growth rate of the sprouts after 3 days of inoculation with the pathogen, the YV148 line was highly resistant to the pathogen. The remaining lines were either partially or fully infected. The highly resistant line YV 148 is suitable for future breeding programs due to their thin sprouts and fast growing nature.
Keywords
Mungbean; Pseudomonas species; resistance screening; sprout rot; 16S rRNA gene sequence;
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