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http://dx.doi.org/10.5352/JLS.2012.22.7.970

Genetic Diversity of Culturable Endophytic Fungi Isolated from Halophytes Naturally Growing in Muan Salt Marsh  

You, Young-Hyun (Department of Life Sciences and Biotechnology, Kyungpook National University)
Yoon, Hyeok-Jun (Department of Life Sciences and Biotechnology, Kyungpook National University)
Seo, Yeong-Gyo (Department of Life Sciences and Biotechnology, Kyungpook National University)
Kim, Mi-Ae (Department of Life Sciences and Biotechnology, Kyungpook National University)
Kang, Myung-Suk (National Institute of Biological Resource)
Kim, Chang-Mu (National Institute of Biological Resource)
Ha, Sang-Chul (Department of Confectionary and Decoration, Daegu Mirae college)
Cho, Ga-Youn (National Institute of Biological Resource)
Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Journal of Life Science / v.22, no.7, 2012 , pp. 970-980 More about this Journal
Abstract
Native halophytes, such as Suaeda maritima, Limonium tetragonum, S. japonica, Zoysia sinica, and Phragmites australis were collected from the Muan salt marsh. Ninety endophytic fungi were isolated from the roots of the collected halophytes. Molecular insights inferred by internal transcribed spacer containing ITS1, 5.8s, and the ITS2 region showed that all the fungal strains belong to ten orders, i.e., Capnodiales (4.44%), Cystofilobasidiales (1.11%), Dothideales (3.33%), Eurotiales (53.33%), Glomerellales (3.33%), Hypocreales (8.89%), Mucorales (1.11%), Pleosporales (15.56%), Sordariales (1.11%), and Trichosphaeriales (1.11%). The rest (6.67%) of all fungal isolates were not identified. Ninety fungal strains were confirmed at the genus level, containing Acremonium, Alternaria, Aspergillus, Aureobasidium, Cephalosporium, Chaetomium, Cladosporium, Colletotrichum, Cryptococcus, Didymella, Dothideomycete, Emericellopsis, Epicoccum, Eupenicillium, Fusarium, Gibberella, Gongronella, Macrophoma, Microsphaeropsis, Nigrospora, Paecilomyces, Paraconiothyrium, Penicillium, Phaeomyces, Phoma, Pleosporales, Purpureocillium, and Talaromyces. Of all the endophytic fungi identified from the various halophytes, Aspergillus and Penicillium of Eurotiales had the highest abundance.
Keywords
Endophytic fungi; genetic diversity; halophyte; salt marsh;
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1 Arnold, E. A., Mejia, L. C., Kyllo, D., Rojas, E., Maynard, Z., Robbins, N. and Herre, E. A. 2003. Fungal endophytes limit pathogen damage in a tropical tree. Proc. Natl. Acad. Sci. USA 100, 15649-15654.   DOI
2 Chapman, V. J. 1974. Salt Marshes and Salt Deserts of the World. In Ecology of Halophytes. pp. 3-22, Academic Press, New York.
3 Gomes, N. C. M., Cleary, D. F. R., Pinto, F. N., Egas, C., Almeida, A., Cunha, A., Mendonça-Hagler, L. C. S. and Smalla, K. 2010. Taking root: enduring effect of rhizosphere bacterial colonization in mangroves. PLoS ONE 5, e14065.   DOI
4 Hamayun, M., Khan, S. A., Khan, M. A., Khan, A. L., Kang, S. M., Kim, S. K., Joo, G. J. and Lee, I. J. 2009. Gibberellin production by pure cultures of a new strain of Aspergillus fumigates. World J. Microbiol. Biotechnol. 25, 1785-1792.   DOI
5 Khan, A. L., Hamayun, M., Ahmad, N., Hussain, J., Kang, S. M., Kim, Y. H., Adnan, M. D., Tang, S., Waqas, M., Radhakrishnan, R., Hwang, Y. H. and Lee, I. J. 2011. Salinity Stress Resistance Offered by Endophytic Fungal Interaction Between Penicillium minioluteum LHL09 and Glycine max. L. J. Microbiol. Biotechnol. 21, 893-902.   DOI
6 Khan, A. L., Hamayun, M., Kang, S. M., Kim, Y. H., Jung, H. Y., Lee, J. H. and Lee, I. J. 2012. Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiol. 12, 3.   DOI
7 Kim, B. S., Oh, H. M., Kang, H., Park, S. and Chun, J. 2004. Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J. Microbiol. Biotechnol. 14, 205-211.
8 Khan, S. A., Hamayun, M., Rim, S. O., Lee, I. J., Seu, J. C., Choo, Y. S., Jin, I. N., Kim, S. D., Lee, I. K. and Kim, J. G. 2008. Isolation of endophytic fungi capable of plant growth promotion from monocots inhibited in the coastal sand dunes of Korea. J. Life Sci. 18, 1355-1359.   DOI
9 Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Lee, I. J. and Kim, J. G. 2009. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J. Microbiol. Biotechnol. 25, 829-833.   DOI   ScienceOn
10 Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diveristy of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Korean J. Mycol. 37, 130-133.   DOI
11 Mohamed, D. J. and Martiny, J. B. 2011. Patterns of fungal diversity and composition along a salinity gradient. ISME J. 5, 379-88.   DOI
12 Park, K. H. and Lee, H. K. 2006. Establishment of the wetland ecosystem information system based on Web-GIS in Gyeongnam region. J. Environ. Res. 6, 99-103.
13 Rodriguez, R. J., Henson, J., Van, V. E., Hoy, M., Wright, L., Beckwith, F., Kim, Y. and Redman, R. S. 2008. Stress tolerance in plants via habitat-adapted symbiosis. ISME J. 2, 404-416.   DOI
14 Pielou, E. C. 1975. Ecological diversity. p 165. John Wiley, New York.
15 Redman, R. S., Sheehan, K. B., Stout, R. G., Rodriguez, R. J. and Henson, J. M. 2002. Thermotolerance conferred to plant host and fungal endophyte during mutualistic symbiosis. Science 298, 1581.   DOI   ScienceOn
16 Rodriguez, R. J., Redman, R. S. and Henson, J. M. 2004. The role of fungal symbioses in the adaptation of plants to high stress environments. Mitigation and Adaptation Strategies for Global Change 9, 261-272.   DOI
17 Seo, S. T., Kim, K. H., Kim, M. J., Hong, J. S., Park, J. H. and Shin, S. C. 2009. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Korean J. Mycol. 37, 108-110.   DOI
18 Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Bio. Evol. 24, 1596-1599.   DOI   ScienceOn
19 Vazquez M. M., Cesar, S., Azcon, R. and Barea, J. M. 2000. Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl. Soil Ecol. 15, 261-272.   DOI   ScienceOn
20 Waller, F., Achatz, B., Baltruscha, T. H., Fodor, J., Becker, K., Fischer, M., Heier, T., Hckelhoven, R., Neumann, C., Wettstein, D. V., Franken, P. and Kogel, K. H. 2005. The endophytic fungus Piriformospora indica reprograms barley to saltstress tolerance, disease resistance, and higher yield. Proc. Natl. Acad. Sci. USA 102, 13386-13391.   DOI   ScienceOn
21 Zhang, X. Y., Bao, J. G., Wang, H., He, F., Xu, X. Y. and Qi, S. H. 2012. Diversity and Antimicrobial activity of culturable fungi isolated from six species of the South China sea gorgonians. Microbiol. Ecol. In press. DOI 10.1007/s00248-012-0050-x.
22 Yamada, A., Takeo, O., Yosuke, D. and Masatake, O. 2001. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience 42, 43-50.   DOI   ScienceOn
23 You, Y. H., Yoon, H., Lee, G. S., Woo, J. R., Shin, J. H., Lee, I. J., Rim, S. O., Choo, Y. S. and Kim, J. G. 2011. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. J. Life Sci. 21, 992-996.   DOI
24 You, Y. H., Yoon, Woo, J. R. Seo, Y., Kim, M. Lee, G. and Kim, J. G. 2012. Diversity of endophytic fungi from the roots of halophytes growing in go-chang salt marsh. Korean J. Mycol. 40, 86-92.   DOI