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http://dx.doi.org/10.5352/JLS.2012.22.7.964

Genistein Suppresses TPA-Induced Matrix Metalloproteinases Activity and Cell Invasion in Human Breast Adenocarcinoma Cells  

Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Department of Biomaterial Control (BK21 program), Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Kim, Sung-Ok (Department of Herbal Pharmacology of Oriental Medicine, College of Oriental Medicine & R&D Team for the New Drug of Oriental Medicine (BK21), Daegu Hanny University)
Publication Information
Journal of Life Science / v.22, no.7, 2012 , pp. 964-969 More about this Journal
Abstract
Genistein, a predominant isoflavone, has been shown to inhibit the growth of various cancer cells in vitro and in vivo without toxicity to normal cells. In the present study, we investigated the effects of genistein on the activity and the expression of matrix metalloproteinases (MMPs) in MCF-7 and MDA-MB-231 human breast adenocarcinoma cells. Our findings showed that MMP-9 and -2 activation was significantly increased in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). However, the increased activities of MMP-9 and -2 in TPA-treated cells were concentration-dependently inhibited by treatment with genistein, and this was also correlated with a decrease in the expression of their mRNA and proteins. In addition, a matrigel invasion assay showed that genistein reduced TPA-induced invasion of MCF-7 and MDA-MB-231 cells. Although further in vivo studies are needed, these results suggest that genistein treatment may inhibit tumor cell invasion and, therefore, act as a dietary source to decrease the risk of cancer metastasis.
Keywords
Genistein; breast cancer; 12-O-tetradecanoyl phorbol-13-acetate (TPA); matrix metalloproteinases (MMPs); invasion;
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