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http://dx.doi.org/10.5352/JLS.2012.22.7.857

Effects of Opuntia humifusa Supplementation on Lipid Peroxidation and SOD Protein Expression in the Liver, Kidney, and Skeletal Muscle of Rats Fed a High-fat Diet

Kwon, Dae-Keun (Laboratory of Sports Nutrition, Sunmoon University)
Kang, Jun-Yong (Laboratory of Sports Nutrition, Sunmoon University)
Park, Jin-Ho (Laboratory of Sports Nutrition, Sunmoon University)
Ryu, Sung-Pil (Department of Leisure Sports, Kyungpook National University)
Song, Young-Ju (Laboratory of Sports Nutrition, Sunmoon University)

Publication Information

Journal of Life Science / v.22, no.7, 2012 , pp. 857-862 More about this Journal

Abstract

This study was conducted to investigate the effects of Opuntia humifusa supplementation on lipid peroxidation and superoxide dismutase (SOD) protein expression at resting state in various organs of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats, 6 weeks of age, were randomly divided into two groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). They were given a high-fat diet (CG) or a diet supplemented with 5% of O. humifusa (EG) for 8 weeks. The results showed that the malondialdehyde (MDA) levels of the kidney and the liver were significantly lower in the EG group than in the CG group (p<0.01). In addition, the MDA levels in the skeletal muscle of the EG group tended to be lower than those in the CG group, but this difference was not significant. The Cu, Zn-SOD protein expression in the kidney of the EG group was significantly increased compared with that of the CG group (p<0.01). The Mn-SOD protein expression in the skeletal muscle of the EG group was significantly increased compared with that of the CG group (p<0.01). These results suggest that O. humifusa supplementation has antioxidative properties, which are exerted in a specific organ manner, and that it inhibits the action of lipid peroxidation and the expression of SOD in rats fed a high-fat diet.

Keywords

Opuntia humifusa; high fat diet; malondialdehyde (MDA); reactive oxygen species (ROS);

Citations & Related Records

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