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http://dx.doi.org/10.5352/JLS.2012.22.6.837

Comparison of Endo-, Exo-Cellular Enzyme Activity for New Strains of Hypsizygus marmoreus  

Lee, Chang-Yun (Greenpeace Mushroom Co.)
Song, Ho-Sung (Greenpeace Mushroom Co.)
Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
Woo, Ju-Ri (Department of Life Sciences and Biotechnology, Kyungpook National University)
You, Young-Hyun (Department of Life Sciences and Biotechnology, Kyungpook National University)
Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Journal of Life Science / v.22, no.6, 2012 , pp. 837-843 More about this Journal
Abstract
This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the $1^{st}$ cultivation experiment, and selected six strains from one of 58 strains through the $2^{nd}$ cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of ${\alpha}$-amylase at the highest as 73.9~102.2 unit/mg protein, and chitinase is lower than ${\alpha}$-amylase at 8.1~13.1 unit/mg protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of ${\alpha}$-amylase is the highest at 5,292~1,184 unit/mg protein, and CMCase and xylanase were 1,140~245 unit/mg protein, 94~575 unit/mg protein, compared to each other. However, the enzyme activity of ${\beta}$-glucosidase and chitinase is low.
Keywords
Hypsizygus marmoreus; ${\alpha}$-amylase; CMCase; enzyme activity; new cultivars;
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