Browse > Article
http://dx.doi.org/10.5352/JLS.2012.22.6.828

Effect of Flammulina velutipes Extracts Cultivated with Oriental Herbal Plants on the Activation of Immune Cells  

Kim, Jong-Jin (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Lee, Sang-Won (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Park, Kyung-Wuk (S&J Bio Co., Ltd., 303 Business Incubator, Sunchon National University)
Seo, Kwon-Il (Department of Food and Nutrition, College of Life Science and Natural Resources, Sunchon National University)
Yee, Sung-Tae (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Publication Information
Journal of Life Science / v.22, no.6, 2012 , pp. 828-836 More about this Journal
Abstract
The objective of the current study was to determine the effects of the extracts isolated from the fruit body of Flammulina velutipes cultivated with oriental herbal plants on mouse splenocytes, B cells, and macrophages in vitro. The ethanol extracts B (EEB) directly induced the proliferation of spleen cells in a dose-dependent manner and increased IL-6, TNF-${\alpha}$, and IFN-${\gamma}$ synthesis. The EEB also increased the proliferation of B cells in a dose-dependent manner. The production of immunoglobulin M, G1, G2a, G2b, and IgG3 in the presence of the EEB increased progressively in the culture supernatant. When the EEB were used in macrophage cell line (RAW264.7) stimulation, there was a marked induction of NO synthesis in a dose-dependent manner and an increased IL-6, TNF-${\alpha}$, and GM-CSF synthesis. Intraperitoneal injection with EBB showed life prolongation effect of 16.1% in mice previously inoculated with sarcoma-180, respectively. These results suggest that the capacity of the EEB isolated from the fruit body of Flammulina velutipes cultivated with oriental herbal plants seems to act as a potent immunomodulator causing augmentation of immune cell activity, and with the absence of notable side-effects, Flammulina velutipes EEB could be used as a biological response modifier having possible therapeutic effects against immunological disorders. This study also showed that functional components of Flammulina velutipes were possibly improved by incorporating oriental herbal plants in a growth medium.
Keywords
Flammulina velutipes; spleeen cells; B cells; macrophage; oriental herbal plants;
Citations & Related Records
Times Cited By KSCI : 13  (Citation Analysis)
연도 인용수 순위
1 Bano, Z. and Rajarathnam, S. 1988. Pleurotus mushrooms. Part II. Chemical composition, nutritional value, post-harvest physiology, preservation, and role as human food. Crit. Rev. Food Sci. Nutr. 27, 87-158.   DOI
2 Choi, S. J., Lee, Y. S., Kim, J. K., Kim, J. K. and Lim, S. D. 2010. Physiological activation of extract from edible mushrooms. J. Korean Soc. Food Sci. Nutr. 39, 1087-1096.   DOI
3 Choi, Y. I., Lee, G. W., Hur, H., Lee, U. Y. and Lee, T. S. 2008. Immno-potentiating and antitumor effects against mouse sarcoma 180 by crude polysaccharides extracted from fruiting body of Russula rosacea. Korean J. Mycol. 36, 84-92.   DOI
4 Cho, N. S., Kim, D. H., Chung, H. C., Lee, S. S., Ohga, S. and Leonowicz, A. 2004. Effect of water soluble fraction from Japanese Larch Wood on Sawdust Cultivation of Lentinula edodes. Mokchae Konghak. 32, 36-44.
5 Han, S. Y., Shon, M. Y. and Lee, S. W. 2003. Physiological activaties of mycelial Flammulina velutipes cultured in liquid grain media. Food Industry Nutr. 8, 50-56.
6 Hayward, A. R., Chmura, K. and Cosyns, M. 2000. Interferon-gamma in required for innate immunity to Crytosporidium pravum in mice. J. Infect. Dis. 182, 1001-1004.   DOI   ScienceOn
7 J., Loh, C. and Rao, A. 1995. Transcriptional regulation of the IL-2 gene. Curr. Opin. Immunol. 7, 333-342.   DOI   ScienceOn
8 Jeurink, P. V., Noguera, C. L., Savelkoul, H. F. and Wichers, H. J. 2008. Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells. Int. Immunopharmacol. 8, 1124-1133.   DOI
9 Jhune, C. S., Sul, H. J., Kong, W. S., Yoo, Y. B., Cheong, J. C. and Chun, S. C. 2006. Effects of NaCl concentration on production and yields of fruiting body of oyster mushrooms, Pleurotus spp. Korean J. Mycol. 34, 39-53.   DOI
10 Kang, H. I., Kim, J. Y., Moon, K. D., Seo, K. I., Cho, Y. S., Lee, S. D. and Yee, S. T. 2004. Effect of the crude polysaccharides of Pleurotus eryngii on the activation of immune cells. J Korean Soc. Food Sci. Nutr. 33, 192-1097.
11 Kim, S. B., Lee, G. W., Lee, U. Y. and Lee, T. S. 2007. Studies on Immuno-Modulatory and Antitumor Effects of crude polysaccharides extracted from fruiting body of Oudemansiella radicata. Korean J. Mycol. 35, 109-114.   DOI
12 Kim, S. B., Lee, G. W., Kim, H. Y., Shim, M. J., Rho, H. S., Lee, H. S., Lee, M. W., Lee, U. Y. and Lee, T. S. 2006. Inhibitive effects of mouse sarcoma 180 by crude polysaccharides extracted from fruiting body of Aranuillaria mellea. Korean J. Mycol. 34, 98-104.   DOI
13 Kim, S. J. and Han, D. S. 2005. Effect of plants extracts on lipid peroxidation of rat brain tissue induced by reactive oxygen species. Korean J. Food Sci Technol. 37, 976-982.
14 Larssn, B. M., Larsson, K., Malmberg, P. and Plamberg, L. 1999. Gram positive bacteria induce IL-6 and IL-8 production in human aveolar macrophages and epithelial cells. Inflammation 23, 217-230.
15 Lee, G. W., Kim, H., Hur, Y. H., Lee, M. W., Shim, M. J., Lee, U. Y. and Lee, T. S. 2008. Antitumor and immuno-modulatoey effect of crude polysaccharides from from fruiting body of Tremella aurantialba. Korean J. Mycol. 36, 66-74.   DOI
16 Lee, N. H., Im, M. H. and Choi, U. K. 2006. Calcium absorption by the fruitbody of Saesongi (Pleurotus eryngii) mushroom. Food Sci. Biotechnol. 15, 308-311.
17 Lee, G. W., Hur, H. H., Lee, U. Y. and Lee, T. S. 2008 Antitumor and Immuno-Modulatory Effect Against Mouse Sarcoma 180 of Crude Polysaccharides Extracts from Fruiting Body of Lentinus giganteus. Korean J. Mycol. 36, 75-83.   DOI
18 Lee, G. W., Kim, H. Y., Lee, U. Y. and Lee, T. S. 2007. Antitumor and immuno-modulatory effect against mouse sarcoma 180 of crude polysaccharides extracts from fruiting body of Armillaria tobescens. Korean J. Mycol. 35, 101-108.   DOI
19 Lee, L. S., Cha, H. S., Park, J. D., Jang, D. J. and Kim, S. H. 2008. Physicochemical properties of mushroom (Flammulina velutipes) cultivated with green tea. J. Korean Soc. Food Sci. Nutr. 37, 190-194.   DOI
20 Lee, S. H., Kwak, W. S. and Kim, W. Y. 2005. Studies on the selenium type and etabolism of selenium accumulation in the selenium-enriched mushroom Flammulina velutipes, and its spend mushroom composts. J. Anim. Sci. Technol. 47, 305-316.   DOI
21 Lee, S. R., Nam, D. Y., Lee, H. J., Park, C. H., Shim, J. C., Lee, U. Y. and Lee, T. S. 2009. Anti-tumor activity of flammulina velutipes extract on B16 cells. Korean J. Food Preserv. 16, 599-603.
22 Li, Y., Ohms, S. J., Shannon, F. M., Sun, C. and Fan, J. Y. 2012. IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages. Biochem. Biophys. Res. Commun. 23, 748-53.
23 Oh, Y. H., Kim, S. B., Lee, G. W., Kim, H. Y., Shim, M. J., Rho, H. S., Lee, H. S., Lee, M. W., Lee, U. Y. and T. S. Lee. 2006. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Tremella Fuciformis. Korean J. Mycol. 34, 105-111.   DOI
24 Yee, S. T., Jeong, Y. R., Ha, M. H., Kim, S. H., Byum, M. W. and Jo, S. K. 2000. Induction of nitric oxide and TNF-$\alpha$ by herbal plant extract in mouse macrophage. J. Korean Soc. Food Sci. Nutr. 29, 342-348.
25 Shim, S. M., Im, K. H., Kim, J. W., Lee, U. Y., Shim, M. J., Lee, M. W. and T. S. Lee. 2003. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Korean J. Mycol. 31, 155-160.   DOI
26 Shim, S. M., Im, K. H., Kim, J. W., Lee, U. Y., Shim, M. J., Lee, M. W. and T. S. Lee. 2003. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from daedaleopsis tricolor. Korean J. Mycol. 31, 161-167.   DOI
27 Wajant, H., Grell, M. and Scheurich, P. 1999. TNF receptor associated factors in cytokine signaling. Cytokine Growth Factor Rev. 10, 15-26.   DOI