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http://dx.doi.org/10.5352/JLS.2012.22.6.807

Study on the Antioxidative and Physiological Activities of Saururus chinensis Extract  

Kang, Chang-Soo (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Lee, Min-Joo (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Park, Cheol-Bem (Fusion Technology, Hoseo University)
Bang, In-Seok (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Publication Information
Journal of Life Science / v.22, no.6, 2012 , pp. 807-814 More about this Journal
Abstract
Saururus chinensis has long been widely used in oriental folk medicines to treat diseases. In the current study, organic solvent fractions obtained from the main methanolic extract of S chinensis were evaluated for their antioxidative and related physiological activities. The antioxidant activity of the fractions was measured using DPPH free radical scavenging activity, increased in a dose-dependent manner, and the $ED_{50}$ of the ethyl acetate fractions exhibited a value of 12.84 ${\mu}g/ml$ higher than 27.22 ${\mu}g/ml$ compared to the BHT. Also, the cell viability of S. chinensis on $H_2O_2$-induced HDF cell death ($IC_{50}$) showed the highest cell viability of 89.39% in 50 ${\mu}g/ml$ of ethyl acetate fraction and 67.98% of visible cell survival rate in n-butanolic fraction. Meanwhile, all fractions of the S. chinensis extract led to a slight down regulation of the mRNA expression of fibulin-5, which is related to skin elasticity, and the ethyl acetate fraction having high antioxidant activity showed a markedly inhibitory effect on chick embryonic angiogenesis using the CAM assay. These results suggest that the ethyl acetate fraction of S. chinensis extract could be a good material in therapeutic application for antioxidant and related anti-angiogenesis activities.
Keywords
Saururus chinensis; antioxidant activity; angiogenesis; CAM assay;
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