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http://dx.doi.org/10.5352/JLS.2012.22.6.751

α-Glucosidase, Tyrosinase, and Elastase Inhibitory Effects of Enzymatic Extracts from Ecklonia cava and its Alcohol Metabolizing Activity  

Kim, Hye-Youn (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Cho, Eun-Kyung (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Kang, Su-Hee (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Bae, Jeong-Mi (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Publication Information
Journal of Life Science / v.22, no.6, 2012 , pp. 751-759 More about this Journal
Abstract
Microbulbifer sp. was used to acquire the degrading products from Ecklonia cava (DPEC) and the products were investigated to determine the physiological activities. Firstly, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity and superoxide dismutase (SOD) assay were about 84.1% and 89.6% at 2.5 mg/ml, respectively. In addition, nitrite scavenging ability was shown to be 56.3% at 0.5 mg/ml on pH 1.2. ${\alpha}$-Glucosidase inhibitory activity was increased in a dose-dependent manner and was about 58.7% at 2.5 mg/ml. To determine the influence of DPEC on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. Facilitating rates of ADH and ALDH activities by DPEC were 123.3% and 215.2% at 2.5 mg/ml, respectively. For analyses of anti-wrinkling and whitening effects, its elastase and tyrosinase inhibitory activities were measured and were about 73.1% and 42.2% at 2.5 mg/ml, respectively. These results indicated that DPEC has valuable biological attributes owing to its antioxidant, nitrite scavenging, and alcohol metabolizing activities and ${\alpha}$-glucosidase, elastase, and tyrosinase inhibitory activities.
Keywords
Microbulbifer sp.; Ecklonia cava; physiological activities; elastase; tyrosinase;
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