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http://dx.doi.org/10.5352/JLS.2012.22.5.627

Biological Analysis of Enzymatic Extracts from Capsosiphon Fulvescens Using the Microbulbifer sp. AJ-3 Marine Bacterium  

Bae, Jeong-Mi (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Cho, Eun-Kyung (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Kim, Hye-Youn (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Kang, Su-Hee (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
Publication Information
Journal of Life Science / v.22, no.5, 2012 , pp. 627-633 More about this Journal
Abstract
$Microbulbifer$ sp. AJ-3 was used to acquire the degrading products from $Capsosiphon$ $fulvescens$ (DPCF), which were investigated to determine its physiological activities. A crude enzyme extract from $Microbulbifer$ sp. AJ-3 hydrolyzes polysaccharide substrates such as agar, agarose, alginic acid, fucoidan, laminaran, starch, and chitin. Among them, agarose, laminaran, and alginic acid showed higher activities, especially alginic acid. The antioxidant activity of DPCF was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and superoxide dismutase (SOD)-like activities and were about 32% and 93% at 2 mg/ml, respectively. In addition, the nitrite-scavenging activity of DPCF was about 82%, 53%, and 12% at pH levels of 1.2, 3.0, and 6.0, respectively. To determine the influence of DPCF on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) was measured. The facilitating rate of ADH activity by DPCF was 130% at 2 mg/ml. The tyrosinase inhibitory activity of DPCF was slightly increased in a dose-dependent manner and was about 28% at 2 mg/ml. These results indicated that the enzymatic products from DPCF have a strong antioxidant, nitrite scavenging, and alcohol metabolizing activity.
Keywords
Capsosiphon fulvescens; polysaccharide-degrading enzymes; enzymatic hydrolysis; antioxidative activity; ADH activity;
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