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http://dx.doi.org/10.5352/JLS.2012.22.5.605

Probiotic Potential of Indigenous Bacillus sp. BCNU 9028 Isolated from Meju  

Shin, Hwa-Jin (Department of Biology, Changwon National University)
Bang, Ji-Hun (Department of Biology, Changwon National University)
Choi, Hye-Jung (Department of Biology and interdisciplinary Program in Biotechnology, Changwon National University)
Kim, Dong-Wan (Department of Microbiology, Changwon National University)
Ahn, Cheol-Soo (Cho-A Pharm. Co, LTD.)
Jeong, Young-Kee (Department of Biotechnology, Dong-A University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Publication Information
Journal of Life Science / v.22, no.5, 2012 , pp. 605-612 More about this Journal
Abstract
Spore-forming bacteria are being used as probiotic supplements for human and animal use, due to their low pH stability and ability to survive the gastric barrier. In this study, the BCNU 9028 strain was screened from meju, a Korean fermented soybean food starter. Biochemical and physiological characteristics, as well as 16S rDNA sequence analyses, indicate that this strain belongs to the genus $Bacillus$. $Bacillus$ sp. BCNU 9028 showed a 92% survivability at pH 2.5 and could also withstand 0.3% ox bile. Furthermore, it was postulated that $Bacillus$ sp. BCNU 9028 could prevent biofilm formation and adherence of food-borne pathogens such as $Listeria$ $monocytogenes$, $S.$ $aureus$ and $E.$ $coli$ on the basis of its autoaggregation and coaggregation capacity with food-borne pathogens. It was shown that BCNU 9028 has good abilities to adhere to the intestinal tract from its hydrophobic character (63.3%). The $Bacillus$ sp. BCNU 9028 strain especially elicited antibacterial activity against both Gram-positive and -negative pathogens. These findings suggested that the $Bacillus$ sp. BCNU 9028 strain could be used as a potential probiotic.
Keywords
Antibacterial activity; Bacillus sp.; coaggregation; Meju; probiotic;
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