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http://dx.doi.org/10.5352/JLS.2012.22.4.559

Regulation of Ethylene Biosynthesis in Phytochrome Mutants of the Arabidopsis Root  

Park, Ji-Hye (Department of Biological Sciences, Andong National University)
Kim, Soon-Young (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.22, no.4, 2012 , pp. 559-564 More about this Journal
Abstract
In order to investigate the effect of phytochromes on the regulation of ethylene biosynthesis, we measured the ethylene production and the activities of enzymes involved in ethylene biosynthesis using phytochrome mutants such as $phyA$, $phyB$, and $phyAB$ of Arabidopsis. The ethylene production was decreased in mutants grown in white light. In particular, double mutants showed a 37% decrease compared to the wild type in ethylene production. When Arabidopsis roots were grown in the dark, mutants did not show a decrease in ethylene production; however, production was significantly decreased in the double mutant grown in red light. Only $phyB$ did not show the decrease in the ethylene production in far-red light. Unlike the ACO activities, the ACS activities of mutants showed the same pattern as the ethylene production under several light conditions. The results of ACS activities confirmed the expression of the ACS gene by RT-PCR analysis. The decrease of ethylene production in mutants was due to the lower activity of ACC synthase, which converts the S-adenosyl-L-methionine (AdoMet) to 1-aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene. These results suggested that both phytochrome A and B play an important role in the regulation of ethylene biosynthesis in Arabidopsis roots in the conversion step of AdoMet to ACC, which is regulated by ACS.
Keywords
Arabidopsis root; phytochrome mutant; ethylene production; light;
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