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http://dx.doi.org/10.5352/JLS.2012.22.4.466

Genetic Diversity and Population Structure of the Scallop Patinopecten yessoensis in Korea, China, and Japan by Random Amplified Polymorphic DNA Markers  

Nam, Myung-Mo (Aquaculture Industry Division, East Sea Fisheries Research Institute)
Lee, Chu (Aquaculture Industry Division, East Sea Fisheries Research Institute)
Moon, Tae-Seok (Aquaculture Industry Division, East Sea Fisheries Research Institute)
Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
Publication Information
Journal of Life Science / v.22, no.4, 2012 , pp. 466-471 More about this Journal
Abstract
Sixty individuals of the scallop $Patinopecten$ $yessoensis$ (Genus Pecten) were sampled to examine the genetic diversity and population structure of this species. Random amplified polymorphic DNA (RAPD) identified 109 genotypes and produced 79 polymorphic loci (72.8%). Total genetic diversity values ($H_T$) and interlocus variation in the within-population genetic diversity ($H_S$) were 0.254 and 0.178, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.299. This indicated that about 70.1% of the total variation was within populations. The unique loci and bands of $P.$ $yessoensis$ were shown in only one population among the three countries. RAPD markers were very effective in classifying the natural population levels of $P.$ $yessoensis$ in Korea, China, and Japan. In addition, insights into the relative gene diversity among and within populations of $P.$ $yessoensis$ would be useful in breeding and for the development of strategies for animal genetic resources.
Keywords
Random amplified polymorphic DNA (RAPD); genetic diversity; $Patinopecten$ $yessoensis$;
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