Browse > Article
http://dx.doi.org/10.5352/JLS.2012.22.3.360

Up-Regulation of NAG-1 and p21 Genes by Sulforaphane  

Jeong, Byung-Geol (Nensys Inc.)
Kim, Soon-Young (Department of Biological Sciences, Andong National University)
Lee, Kon-Joo (Department of Biological Sciences, Andong National University)
Kim, Jong-Sik (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.22, no.3, 2012 , pp. 360-365 More about this Journal
Abstract
We investigated the anti-proliferative activity of sulforaphane and expression changes of NAG-1 and p21 genes in response to sulforaphane treatment in human colorectal HCT116 cells. The results showed that sulforaphane decreased cell viabilities in a dose-dependent manner and induced expression of NAG-1 and p21 proteins in a dose-dependent and time-dependent manner. In addition, we found that NAG-1 expression by sulforaphane was not dependent on the presence of p53, whereas p21 expression was dependent on p53 presence. The results indicated that up-regulation of NAG-1 was not related with the activity of a dietary histone deacetylase inhibitor of sulforaphane. ATF3 induction was detected from 2 hr after sulforaphane treatment, indicating that ATF3 could be a transcription factor to up-regulate NAG-1 expression. The results of this study may help to increase our understanding of the molecular mechanism of anti-cancer activity mediated by sulforaphane in human colorectal cancer cells.
Keywords
Sulforaphane; anti-proliferative activity; NAG-1; p21; colorectal cancer cell;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Traka, M. H., K. F. Chambers, E. K. Lund, R. A. Goodlad, I. T. Johnson, and R. F. Mithen. 2009. Involvement of KLF4 in sulforaphane- and iberin-mediated induction of p21(waf1/cip1). Nutr. Cancer 61, 137-145.   DOI   ScienceOn
2 Wilson, L. C., S. J. Baek, A. Call, and T. E. Eling. Nonsteroidal anti-inflammatory drug-activated gene (NAG-1) is induced by genistein through the expression of p53 in colorectal cancer cells. Int. J. Cancer 105, 747-753.   DOI
3 Yamaguchi, K., S. H. Lee, T. E. Eling, and S. J. Baek. 2006. A novel peroxisome proliferator-activated receptor gamma ligand, MCC-555, induces apoptosis via posttranscriptional regulation of NAG-1 in colorectal cancer cells. Mol. Cancer Ther. 5, 1352-1361.   DOI   ScienceOn
4 Yoshioka, H., H. Kamitani, T. Watanabe, and T. E. Eling. 2008. Nonsteroidal anti-inflammatory drug-activated gene (NAG-1/GDF15) expression is increased by the histone deacetylase inhibitor trichostatin A. J. Biol. Chem. 283, 33129-33137.   DOI
5 Jang, M. J., H. E. Kim, S. M. Son, M. J. Kim, E. W. Seo, Y. H. Kim, and J. S. Kim. 2009. Over-expression of NSAID activated gene-1 by caffeic acid phenethyl ester. J. Life Sci. 19, 1787-1793.   DOI
6 Kim, J. S., S. J. Baek, T. Sali, and T. E. Eling. 2005. The conventional nonsteroidal anti-inflammatory drug sulindac sulfide arrests ovarian cancer cell growth via the expression of NAG-1/MIC-1/GDF-15. Mol. Cancer Ther. 4, 487-493.
7 Lee, S. H., J. S. Kim, K. Yamaguchi, T. E. Eling, and S. J. Baek. 2005. Indole-3-carbinol and 3,3'-diindolylmethane induce expression of NAG-1 in a p53-independent manner. Biochem. Biophys. Res. Commun. 328, 63-69.   DOI   ScienceOn
8 Lee, S. H., K. Yamaguchi, J. S. Kim, T. E. Eling, S. Safe, Y. Park, and S. J. Baek. 2006. Conjugated linoleic acid stimulates an anti-tumorigenic protein NAG-1 in an isomer specific manner. Carcinogenesis 27, 972-981.   DOI   ScienceOn
9 Li, Y., T. Zhang, H. Korkaya, S. Liu, H. F. Lee, B. Newman, Y. Yu, S. G. Clouthier, S. J. Schwartz, M. S. Wicha, and D. Sun. 2010. Sulforaphane, a dietary component of broccoli/ broccoli sprouts, inhibits breast cancer stem cells. Clin. Cancer Res. 16, 2580-2590.   DOI
10 Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402-408.   DOI   ScienceOn
11 Meeran, S. M., A. Ahmed, and T. O. Tollefsbol. 2010. Epigenetic targets of bioactive dietary components for cancer prevention and therapy. Clin. Epigenetics 1, 101-116.   DOI   ScienceOn
12 Baek, S. J., J. S. Kim, F. R. Jackson, T. E. Eling, M. F. McEntee, and S. H. Lee. 2004. Epicatechin gallate-induced expression of NAG-1 is associated with growth inhibition and apoptosis in colon cancer cells. Carcinogenesis 25, 2425-2432.   DOI
13 Piyanuch, R., M. Sukhthankar, G. Wandee, and S. J. Baek. Berberine, a natural isoquinoline alkaloid, induces NAG-1 and ATF3 expression in human colorectal cancer cells. Cancer Lett. 258, 230-240.   DOI
14 Sun, X., L. Mi, J. Liu, L. Song, F. L. Chung, and N. Gan. 2011. Sulforaphane prevents microcystin-LR-induced oxidative damage and apoptosis in BALB/c mice. Toxicol. Appl. Pharmacol. 255, 9-17.   DOI   ScienceOn
15 Baek, S. J., L. C. Wilson, and T. E. Eling. 2002. Resveratrol enhances the expression of non-steroidal anti-inflammatory drug-activated gene (NAG-1) by increasing the expression of p53. Carcinogenesis 23, 425-434.   DOI
16 Baek, S. J., R. Okazaki, S. H. Lee, J. Martinez, J. S. Kim, K. Yamaguchi, Y. Mishina, D. W. Martin, A. Shoieb, M. F. McEntee, and T. E. Eling. 2006. Nonsteroidal anti-inflammatory drug-activated gene-1 over expression in transgenic mice suppresses intestinal neoplasia. Gastroenterology 131, 1553-1560.   DOI   ScienceOn
17 Berner, C., E. Aumüller, A. Gnauck, M. Nestelberger, A. Just, and A. G. Haslberger. 2010. Epigenetic control of estrogen receptor expression and tumor suppressor genes is modulated by bioactive food compounds. Ann. Nutr. Metab. 57, 183-189.   DOI   ScienceOn
18 Brandenburg, L. O., M. Kipp, R. Lucius, T. Pufe, and C. J. Wruck. 2010. Sulforaphane suppresses LPS-induced inflammation in primary rat microglia. Inflamm. Res. 59, 443-450.   DOI   ScienceOn
19 Cekanova, M., S. H. Lee, R. L. Donnell, M. Sukhthankar, T. E. Eling, S. M. Fischer, and S. J. Baek. 2009. Nonsteroidal anti-inflammatory drug-activated gene-1 expression inhibits urethane-induced pulmonary tumorigenesis in transgenic mice. Cancer Prev. Res. 2, 450-458.   DOI   ScienceOn
20 Dashwood, R. H. and E. Ho. 2007. Dietary histone deacetylase inhibitors: from cells to mice to man. Semin. Cancer Biol. 17, 363-369.   DOI   ScienceOn
21 Fahey, J. W., Y. Zhang, and P. Talalay. 1997. Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc. Natl. Acad. Sci. USA 94, 10367-10372.   DOI   ScienceOn
22 Ho, E., J. D. Clarke, and R. H. Dashwood. 2009. Dietary sulforaphane, a histone deacetylase inhibitor for cancer prevention. J. Nutr. 139, 2393-2396.   DOI   ScienceOn
23 Baek, S. J., K. S. Kim, J. B. Nixon, L. C. Wilson, and T. E. Eling. 2001. Cyclooxygenase inhibitors regulate the expression of a TGF-beta superfamily member that has proapoptotic and antitumorigenic activities. Mol. Pharmacol. 59, 901-908.