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http://dx.doi.org/10.5352/JLS.2012.22.2.133

TGF-β1 Expression by Proliferated Keratinocytes in the Skin of E-Irradiated Mice  

Yoon, A-Ran (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Kim, Do-Nyun (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Seo, Min-Koo (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Oh, Sang-Taek (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Seo, Jung-Seon (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Jun, Se-Mo (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Cha, Jung-Ho (Department of Anatomy, College of Medicine, The Catholic University of Korea)
Lee, Seung-Deok (Department of Oriental Medicine, The Graduate School of Dongguk University)
Lee, Suk-Kyeong (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Publication Information
Journal of Life Science / v.22, no.2, 2012 , pp. 133-141 More about this Journal
Abstract
In this study, we established a radiodermatitis animal model and investigated the change in immune cell proportions in the secondary lymphoid organs. The cells responsible for the increased transforming growth factor-${\beta}1$ (TGF-${\beta}1$) and interleukin-10 (IL-10) production in the lesions following irradiation were also investigated. The radiodermatitis model was constructed by locally exposing the posterior dorsal region of hairless-1 (HR-1) mice to 10 Gy electron (E)-ray/day for six consecutive days. The change in immune cell proportions was analyzed by FACS. Immunohistochemistry was carried out to detect the expression of cytokines and cell-specific markers in the skin. The proportions of antigen-presenting cells, T cells, and B cells in the lymph nodes and spleen were affected by E-irradiation. After irradiation, TGF-${\beta}1$ and IL-17 were co-localized in the papillary region of the dermis with keratin-14 (K-14)-positive cells rather than with regulatory T cells (Treg). IL-10 was not co-stained with Treg, T helper 17 (Th17) cells, dendritic cells, or macrophages. Our data indicate that TGF-${\beta}1$ is over-expressed mainly by proliferated keratinocytes in the lesions of a radiodermatitis animal model.
Keywords
Transforming growth factor-${\beta}1$; keratinocyte; radiodermatitis; interlukin-10; regulatory T cell;
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